- Peptide (C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2 (Accession P12336). Intracellular, C-terminus.
- Expression of Glucose Transporter 2 in rat and mouse brainImmunohistochemical staining of rat and mouse brain sections using Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody (#AGT-022-AG). A. GLUT2 staining (green) in rat dentate gyrus is detected in cells of the granule layer (G) and in the hilus (arrows). B. In mouse hippocampal CA3 region, GLUT2 staining (green) appears in cells of the pyramidal layer. DAPI counterstain (blue) is used to stain nuclei.
The GLUT protein family is encoded by SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, in addition to several conserved residues and motifs designated “sugar transporter signatures”.
Fourteen GLUT isoforms have been identified and divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT2 together with GLUT1, 3, 4 and 14 belongs to Class I group1.
GLUT2 acts as a facilitative glucose transporter in the liver, pancreas, intestine, kidney, and brain and is able to process high sugar concentrations. Due to the transporter’s low affinity and high capacity, it guaranties large bidirectional fluxes of glucose in and out of the cell. GLUT2 also can transport other dietary sugars including fructose and galactose when they are present in physiological concentrations2.
GLUT2 mutations cause a severe and rare syndrome called Fanconi-Bickel, an autosomal recessive disorder in carbohydrate metabolism. Patients with Fanconi-Bickel disorder suffer from hepatomegaly, nephropathy, fasting hypoglycemia, sugar intolerance, and growth retardation. In addition, a GLUT2 polymorphism has been associated with preferences for sugary food2.
Species reactivity key:
Anti-GLUT2 (SLC2A2) Antibody (#AGT-022) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 2 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT2 from human, mouse, and rat samples.
Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody (#AGT-022-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.