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Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody

Glucose transporter 2, Facilitated glucose transporter member 2

Cat #: AGT-022-AG
Alternative Name Glucose transporter 2, Facilitated glucose transporter member 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2 (Accession P12336). Intracellular, C-terminus.
Accession (Uniprot) Number P12336
Gene ID 25351
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 8/13 amino acid residues identical.
RRID AB_2756643.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody #AGT-022), and immunohistochemical staining (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
  • Expression of Glucose Transporter 2 in rat and mouse brain
    Expression of Glucose Transporter 2 in rat and mouse brain
    Immunohistochemical staining of rat and mouse brain sections using Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody (#AGT-022-AG). A. GLUT2 staining (green) in rat dentate gyrus is detected in cells of the granule layer (G) and in the hilus (arrows). B. In mouse hippocampal CA3 region, GLUT2 staining (green) appears in cells of the pyramidal layer. DAPI counterstain (blue) is used to stain nuclei.
References
  1. Lacombe, V.A. (2014) ISRN Vet Sci. 2014, 409547.
  2. Leturque, A. et al. (2009) Am. J. Physiol. Endocrinol. Metab. 296, E985.
Scientific background

The GLUT protein family is encoded by SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, in addition to several conserved residues and motifs designated “sugar transporter signatures”.

Fourteen GLUT isoforms have been identified and divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT2 together with GLUT1, 3, 4 and 14 belongs to Class I group1.

GLUT2 acts as a facilitative glucose transporter in the liver, pancreas, intestine, kidney, and brain and is able to process high sugar concentrations. Due to the transporter’s low affinity and high capacity, it guaranties large bidirectional fluxes of glucose in and out of the cell. GLUT2 also can transport other dietary sugars including fructose and galactose when they are present in physiological concentrations2.

GLUT2 mutations cause a severe and rare syndrome called Fanconi-Bickel, an autosomal recessive disorder in carbohydrate metabolism. Patients with Fanconi-Bickel disorder suffer from hepatomegaly, nephropathy, fasting hypoglycemia, sugar intolerance, and growth retardation. In addition, a GLUT2 polymorphism has been associated with preferences for sugary food2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-GLUT2 (SLC2A2) Antibody (#AGT-022) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 2 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT2 from human, mouse, and rat samples.

Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody (#AGT-022-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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