Anti-Glucose Transporter 3 (GLUT3) (extracellular)-FITC Antibody

Facilitated glucose transporter member 3, SLC2A3
    Cat #: AGT-023-F
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide (C)KDFLNYTLEERLED, corresponding to amino acid residues 39 - 52 of rat Glucose Transporter 3 (Accession Q07647). Extracellular, 1st loop.
    Accession (Uniprot) Number Q07647
    Gene ID 25551
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize human GLUT3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGT-023), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
    Direct flow cytometry
    Direct flow cytometry of live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Glucose Transporter 3 (GLUT3) (extracellular)-FITC Antibody (#AGT-023-F), (5 µg).
    References
    1. Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
    2. Simpson, I.A. et al. (2008) Am J. Physiol. 295, E242.
    3. Zheng, C. et al. (2016) Oncol. Lett. 12, 125.
    Scientific background

    The GLUT protein family is encoded by the SLC2 genes and are a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics1,2.

    GLUT3 is a low-Km protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called “neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH2- and COOH- termini. In addition, there are several conserved residues and motifs designated “sugar transporter signatures”1,2.

    GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/08/2018

    Anti-Glucose Transporter 3 (GLUT3) (extracellular) Antibody (#AGT-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from rat and mouse samples. It will not recognize GLUT3 from human samples.

    Anti-Glucose Transporter 3 (GLUT3) (extracellular)-FITC Antibody (#AGT-023-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

    For research purposes only, not for human use