Anti-GRIP1 Antibody

Glutamate receptor interacting protein 1
    Cat #: APZ-015
    Alternative Name Glutamate receptor interacting protein 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RKSVTLRKMKQEIKE, corresponding to amino acid residues 963-977 of rat GRIP1 (Accession P97879). Intracellular, C-terminus.
        Anti-GRIP1 Antibody
    Accession (Uniprot) Number P97879
    Gene ID 84016
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    RRID AB_2756774.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
      • Anti-GRIP1 Antibody
        Western blot analysis of mouse brain lysate:
        1. Anti-GRIP1 Antibody (#APZ-015), (1:200).
        2. Anti-GRIP1 Antibody, preincubated with the negative control antigen.
        Anti-GRIP1 Antibody
        Western blot analysis of rat brain synaptosomal fraction:
        1. Anti-GRIP1 Antibody (#APZ-015), (1:200).
        2. Anti-GRIP1 Antibody, preincubated with the negative control antigen.
    References
      • GRIP1 (glutamate receptor interacting protein 1) is a neuron-enriched scaffolding protein that plays an important role in regulating synaptic trafficking, synaptic strength and synaptic organization and transmission of AMPA receptor 2 and 3 (GluA2/3) 1,2. GRIP1 structure contains 7 PDZ domains responsible for protein-protein interactions. GRIP1 forms homodimers that can bind and simultaneously organize and assemble protein complexes via PDZ domains1,2.

        GRIP1 is abundantly expressed in glutamatergic and GABAergic synapses. In addition, GRIP1 expression can be found in muscle cells and in the epidermis1,3. Studies on GRIP1 knockout mice have revealed multi-organ developmental abnormalities. These mice die due to hemorrhage and hypovolemia in the embryonic or early post-natal period2.

        Mutations in GRIP1 protein resulting in a gain-of-function phenotype are linked to autism, while neuronal-specific loss-of-function mice have prosocial behavior. Other point mutations have been associated with schizophrenia and congenital kidney disease2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 15/08/2019

    Anti-GRIP1 Antibody (#APZ-015) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GRIP1 from human, mouse, and rat samples.

    For research purposes only, not for human use