Anti-SynGAP Antibody

Synaptic Ras GTPase Activating Protein 1, Synaptic Ras-GAP 1, SYNGAP1
    Cat #: APZ-032
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KSMDESRLDRVKEYE, corresponding to amino acid residues 1185-1199 of rat SynGAP (Accession Q9QUH6). C-terminus.
    Accession (Uniprot) Number Q9QUH6
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ip
    Western blot
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): 
    1, 2 Anti-SynGAP Antibody (#APZ-032), (1:400).
    3, 4. Anti-SynGAP Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of SynGAP in mouse globus pallidus and rat lateral septum
    Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections using Anti-SynGAP Antibody (#APZ-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. SynGAP staining (green) in mouse brain is detected in neuropil (arrows) in the globus pallidus (GP) that is markedly stronger than in the striatum. B. SynGAP staining in rat brain sections shows lateral septum labeling of profiles of neuronal processes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Jeyabalan, N. and Clement, J.P. (2016) Front. Cell. Neurosci. 10, 32.
    Scientific background

    SynGAP1 is a major signaling protein that plays a pivotal role in regulating fundamental molecular changes in dendritic spine synaptic morphological and functional modifications.

    SynGAP1 functions as a downstream component of NMDA receptor-associated signaling and of AMPA receptor trafficking to excitatory postsynaptic membrane. SynGAP1 is mainly expressed in the hippocampus and cortex.

    SynGAP1 is a cytosolic protein without a signal peptide or any transmembrane domain. The N-terminal half of SYNGAP1 has a Ras-GAP domain, along with the region that is loosely homologous to Pleckstrin homology and a C2 domain which is potentially involved in binding Ca2+ and phospholipids. Interestingly, the alignment of GAP domain of SynGAP1 with other Ras-GAPs suggests that the amino acids in the GAP domain are crucial for interacting with Ras and for stimulating Ras-GTPase activity. The C-terminal half consists of a repeat of 10 histidines that may be involved in metal chelation, several potential serine and tyrosine phosphorylation sites and a T/SXV motif necessary for interaction with SAP102 and PSD-95.

    SynGAP1 has been implicated in the pathogenesis of intellectual disability.  de novo out-of-frame deletions were identified in patients with intellectual disability presenting with microcephaly and generalized epilepsy while de novo microdeletions in chromosome region 6p21.3 were observed in patients with severe language impairment in addition to epilepsy. SynGAP1 may also be involved in the pathogenesis of schizophrenia through altered regulation of the NMDA receptor complex.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:


    Immuno-colocalization of GluN2A and SynGAP in rat cingulate cortex.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2A (GluN2A) (extracellular)-ATTO-488 Antibody (#AGC-002-AG), (1:200) and Anti-SynGAP Antibody (#APZ-032), (1:200), followed by donkey-anti-rabbit-Cy3. A. GluN2A staining (green) appears in neuronal profiles (arrows). B. SynGAP staining (red) is detected mostly in apical dendrites (horizontal arrows) and in neuronal soma (vertical arrows). C. Merge of the two images demonstrates colocalization in some neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).

    Last update: 29/10/2018

    Anti-SynGAP Antibody (#APZ-032) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SynGAP from human, mouse, and rat samples.

    For research purposes only, not for human use