Anti-GluR2 (GluA2) (extracellular) Antibody

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2
Cat #: AGC-005
Alternative Name AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
      • Peptide NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2 (Accession P19491). Extracellular, N-terminus.
    Accession (Uniprot) Number P19491
    Gene ID 29627
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 14/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, lci, wb
    May also work in: ifc
      • Western blot analysis of rat brain membranes:
        1. Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005), (1:200).
        2. Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Immunoprecipitation of rat brain lysates:
        1. Brain lysates + protein A beads + Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005).
        2. Brain lysates + protein A beads + pre-immune rabbit serum.
        3. Brain lysates.

        Black arrow indicates the GluR2 protein while the red arrow shows the IgG heavy chain. The immunoblot was performed with Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005).
      • Expression of AMPA Receptor 2 (GluA2) in rat cerebellum
        Immnunohistochemical staining of rat cerebellum using Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) . A. Co-localization of GluA2 (green) and calbindin D28-K (red) in Purkinje cell dendrites (vertical arrow). B. Distribution of calbindin D28-K. C. Distribution of GluA2. Note that some GluA2 positive fibers (horizontal arrow) that are probable Bergmann glial processes do not stain for GluA2. D. The molecular and Purkinje layer (blue).
      • AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.

        The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.

        The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.

        AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2. The Ca2+ permeability of AMPA2 subunit is determined by the presence of the amino acid arginine (R) at a critical site in the pore loop instead of glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.

        Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.

        Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.

        Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/06/2019

    Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 2. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR2 from human, mouse, and rat samples.

    For research purposes only, not for human use
    Citations
      • Immunohistochemical staining of mouse brain sections. Tested in GLUA2-/- mice.
        Egbenya, D.L. et al. (2018) Mol. Cell. Neurosci. 92, 93.
      • Rat brain lysate (1:500).
        Szczurowska, E. et al. (2016) Exp. Neurol. 283, 97.
      • Rat medial prefrontal cortex (mPFC) (1:2000).
        Caffino, L. et al. (2015) Addict. Biol. 20, 158.
      • Rat retinal lysate.
        Dong, L.D. et al. (2015) J. Neurosci. 35, 5409.
      • Mouse brain lysate.
        Khoutorsky, A. et al. (2015) eLife 4, e12002.

      • Rat brain lysate (1:1000).
        Caffino, L. et al. (2014) Pharmacol. Rep. 66, 198.
      • Mouse brain lysate.
        Hamada, S. et al. (2014) Eur. J. Neurosci. 40, 3136.

      • Rat cortical lysate (1:5000).
        Banerjee, B. et al. (2013) Neurogastroenterol. Motil. 25, 973.
      • Mouse brain sections. Also tested in GLUA2-/- mice.
        Egbenya, D.L. et al. (2018) Mol. Cell. Neurosci. 92, 93.

      • Rat brain sections.
        Haglerod, C. et al. (2017) Neuroscience 344, 102.
      • Rat retinal sections (1:200).
        Dong, L.D. et al. (2015) J. Neurosci. 35, 5409.
      • Rat brain sections.
        Ganea, D.A. et al. (2015) Neuropsychopharmacology 40, 2727.
      • Fourie, C. et al. (2014) J. Neurodegener. Dis. 2014, 938530.
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