- Peptide (C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3 (Accession Q9JKA8). Intracellular, C-terminus.
- Western blot analysis of HCN3 in rat brain lysate:1. Anti-HCN3 Antibody (#APC-057), (1:200).
2. Anti-HCN3 Antibody, preincubated with HCN3 Blocking Peptide (#BLP-PC057).
- Expression of HCN3 in rat dorsal root ganglia (DRG)Immunohistochemical staining of frozen section of rat DRG using Anti-HCN3 Antibody (#APC-057), (1:50) followed by mouse anti-rabbit Alexa Fluor 555 (red). Staining is present in neuronal cell bodies. Hoechst 33342 (blue) is used as counterstain.
Hyperpolarization-activated cation currents (Ih) appear in the heart and brain and play a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2
The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2
The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2
The channels are closely related to each other and share homology of about 60%. However, they differ from each other in their cytoplasmic N- and the C-terminus. HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3
The HCN3 channels are abundant in the brain where high expression of HCN3 mRNA was found in the hypothalamus.
Species reactivity key:
Anti-HCN3 Antibody (#APC-057) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN3 from human, rat, and mouse samples.
- Rat diaphragm sections (1:25).
Negrini, D. et al. (2016) Am. J. Physiol. 311, H892.