This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
- Peptide (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2 (Accession O88801). Intracellular, C-terminus.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat hippocampus lysate (lanes 2 and 4):1,2. Anti-Homer2 Antibody (#APZ-027), (1:200).
3,4. Anti-Homer2 Antibody, preincubated with the negative control antigen.Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):1,2. Anti-Homer2 Antibody (#APZ-027), (1:200).
3,4. Anti-Homer2 Antibody, preincubated with the negative control antigen.
- Expression of Homer2 in mouse cerebellum and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-Homer2 Antibody (#APZ-027), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. Homer2 staining (green) in mouse cerebellum is observed in Purkinje cells (arrows) and their dendrites. B. Homer2 staining (green) in rat hippocampal dentate gyrus region is detected granule layer (GL) and interneurons in the hilus (arrows). Cell nuclei are stained with DAPI (blue).
Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.
The Homer family includes three members, Homer1b/c, Homer2a/b, and Homer3. Homer2 protein, also called Vesl-2, is a scaffolding protein that is important for the proper organization, localization and signaling of excitatory postsynaptic receptors. Homer2 protein associates with filamentous actin (F-actin) and directly binds to drebrin, an actin-binding and remodeling protein in dendritic spines. The protein also binds to activated Cdc42, a member of the Rho small GTPase family2. The protein interacts with mGluR1, mGluR5, PI3 Kinase enhancer (PIKE), inositol 1,4,5-trisphosphate receptors (IP3), SHANK and other proteins3,4.
Deletion of the Homer2 gene in mice demonstrates several abnormalities in glutamatergic signaling and in the nucleus accumbens such as reduced basal extracellular glutamate content and reduced function and total protein expression of both mGluR1 and the cystine-glutamate exchanger4.
Species reactivity key:
Anti-Homer2 Antibody (#APZ-027) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer2 from human, mouse, and rat samples.
- Anti-Homer1 Antibody (#APZ-026)
- Anti-mGluR1 (extracellular) Antibody (#AGC-006)
- Anti-mGluR5 (extracellular) Antibody (#AGC-007)
- Anti-PSD-95 Antibody (#APZ-009)
- Anti-Shank1 Antibody (#APZ-011)
- Anti-Shank3 Antibody (#APZ-013)
- Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019)
- Anti-Ryanodine Receptor 1 Antibody (#ARR-001)
- Explorer kits