Free shipping starts now, no minimum, no coupons required!

Anti-Human CX3CR1 (extracellular)-FITC Antibody

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
Cat #: ACR-059-F
Alternative Name Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)ESVTENFEYDDLAE, corresponding to amino acid residues 6-19 of human CX3CR1 (Accession P49238). Extracellular, N-terminus.
Accession (Uniprot) Number P49238
Gene ID 1524
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only
RRID AB_2876847
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The antibody will not recognize the protein from rat or mouse samples.
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-059), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of CX3CR1 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of CX3CR1 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Human CX3CR1 (extracellular)-FITC Antibody (#ACR-059-F), (2.5 µg).
  • Cell surface detection of CX3CR1 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of CX3CR1 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Human CX3CR1 (extracellular)-FITC Antibody (#ACR-059-F), (2.5 µg).
References
  1. Bronger, H. et al. (2019) Cancer Metastasis Rev. 38, 417.
  2. Hamon, P. et al. (2017) Blood 129, 1296.
  3. Luo, P. et al. (2019) Brain. Res. Bull. 146, 12.
  4. Lee, M. et al. (2018) Immune Netw. 18, e5.
  5. Asai, H. et al. (2015) Nat. Neurosci. 18, 1584.
Scientific background

CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.

CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).

Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Widget not in any sidebars
Last update: 20/10/2020

Anti-Human CX3CR1 (extracellular) Antibody (#ACR-059) is a highly specific antibody directed against an epitope of human CX3C chemokine receptor 1. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CX3CR1 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

Anti-Human CX3CR1 (extracellular)-FITC Antibody (#ACR-059-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information