Anti-Human CX3CR1 (extracellular) Antibody

CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • New
Cat #: ACR-059
Alternative Name CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
      • Peptide (C)ESVTENFEYDDLAE, corresponding to amino acid residues 6-19 of human CX3CR1 (Accession P49238). Extracellular, N-terminus.
        Anti-Human CX3CR1 (extracellular) Antibody
    Accession (Uniprot) Number P49238
    Gene ID 1524
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody will not recognize the protein from rat or mouse samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
      • Anti-Human CX3CR1 (extracellular) Antibody
        Western blot analysis of human NK-92MI natural killer cells (lanes 1 and 3) and human THP-1 acute monocytic leukemia (lanes 2 and 4) cell lysates:
        1,2. Anti-Human CX3CR1 (extracellular) Antibody (1:600), (#ACR-059).
        3,4. Anti-Human CX3CR1 (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-Human CX3CR1 (extracellular) Antibody
        Cell surface detection of CX3CR1 in human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + FITC-conjugated goat anti-rabbit antibody.
        ___ Cells + Anti-Human CX3CR1 (extracellular) Antibody (#ACR-059) (2.5 µg/5x105 cells) +FITC-conjugated goat anti-rabbit antibody.
    References
    • 1. Bronger, H. et al. (2019) Cancer Metastasis Rev. doi: 10.1007/s10555-019-09807-3.
    • 2. Hamon, P. et al. (2017) Blood 129, 1296.
    • 3. Luo, P. et al. (2019) Brain. Res. Bull. 146, 12.
    • 4. Lee, M. et al. (2018) Immune Netw. 18, e5.
    • 5. Asai, H. et al. (2015) Nat. Neurosci. 18, 1584.
      • CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.

        CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).

        Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/11/2019

    Anti-Human CX3CR1 (extracellular) Antibody (#ACR-059) is a highly specific antibody directed against an epitope of human CX3C chemokine receptor 1. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CX3CR1 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

    For research purposes only, not for human use