Anti-Human LPAR5 (extracellular) Antibody

Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93
    Cat #: ALR-035
    Alternative Name Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
      • Peptide (C)RSKLVAASVPARDRVR, corresponding to amino acid residues 261-276 of human LPA5 receptor (Accession Q9H1C0). 3rd extracellular loop.
        Anti-Human LPAR5 (extracellular) Antibody
    Accession (Uniprot) Number Q9H1C0
    Gene ID 57121
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, Rat – not recommended for use with mouse and rat samples.
    RRID AB_2756662.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Will not recognize rat or mouse LPA5 receptor.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ifc*, ih*, ip*, lci*
      • Anti-Human LPAR5 (extracellular) Antibody
        Western blot analysis of human chronic myelogenous leukemia MEG-01 (lanes 1 and 3) and K562 cell lysates (lanes 2 and 4):
        1,2. Anti-Human LPAR5 (extracellular) Antibody (#ALR-035), (1:200).
        3,4. Anti-Human LPAR5 (extracellular) Antibody, preincubated with the negative control antigen.
    References
    • 1. Yanagida, K. et al. (2013) Biochim. Biophys. Acta 1831, 33.
    • 2. Amisten, S. et al. (2008) Thromb. Res. 122, 47.
    • 3. Mutoh, T. et al. (2012) Br. J. Pharmacol. 165, 829.
    • 4. Lee, C.W. et al. (2006) J. Biol. Chem. 281, 23589.
    • 5. Jongsma, M. et al. (2012) PLoS ONE 6, e29260.
    • 6. Lin, M.E. et al. (2012) J. Biol. Chem. 287, 17608.
      • LPA (lysophosphatidic acid) mediates cellular differentiation, growth, survival and motility through two subgroups of G-protein coupled receptors (GPCRs): The Edg (endothelial differentiation gene) family – LPA1, LPA2, LPA3 and the non-Edg family – LPA4, LPA5, LPA61.

        Like all GPCRs, LPA receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail1.

        Alkyl-LPA, commonly found in platelets activates LPA5, thus explaining the high expression of the receptor in platelets2. LPA is expressed in astrocytes, and sensory and motor neurons in the spinal cord. It is also detected in spleen, heart, placenta, lliver and colon3.

        LPA5 activation leads to cAMP production; however, it does so in a Gs independent manner4,5. The receptor couples Gq, thus leading to Ca2+ mobilization4.

        Activation of LPA5 was also shown to initiate neuropathic pain in mouse models. LPA5 knockout mice do not develop neuropathic pain6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human LPA5 receptor. Anti-Human LPAR5 (extracellular) Antibody (#ALR-035) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA5 receptor from human samples only.

    For research purposes only, not for human use