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Anti-KCNK10 (TREK-2) Antibody

Potassium channel subfamily K member 10, K2P10.1, TWIK-related K+ channel 2
Cat #: APC-055
Alternative Name Potassium channel subfamily K member 10, K2P10.1, TWIK-related K+ channel 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
Immunogen
  • Peptide TFRNYSLDEEKKEDETEKMC, corresponding to amino acid residues 475-494 of rat KCNK10 (Accession Q9JIS4). Intracellular, C-terminal domain.
Accession (Uniprot) Number Q9JIS4
Gene ID 65272
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - 19/20 amino acid residues identical.
RRID AB_2040138.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat cerebellum lysate:
    Western blot analysis of rat cerebellum lysate:
    1. Anti-KCNK10 (TREK-2) Antibody (#APC-055), (1:200).   
    2. Anti-KCNK10 (TREK-2) Antibody, preincubated with KCNK10/TREK-2 Blocking Peptide (#BLP-PC055).
Immunohistochemistry
  • Rat brain sections.
Immunocytochemistry
  • HEK-293 cells transfected with TREK-2 (1:500) (Acosta, C. et al. (2014) J. Neurosci. 34, 1494.).
References
  1. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648.
  2. Lesage, F. et al. (2000) J. Biol. Chem. 275, 28398.
  3. Bang, H. et al. (2000) J. Biol. Chem. 275, 17412.
Scientific background

KCNK10 (TREK-2, K2P10.1) is a member of the 2-pore (2P) domain K+ channels. These K+ channels are also known as background or “leak” channels and help to set the resting membrane potential.

K2P channels can be activated by a wide variety of stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can also be subclassified based on their specific activators.  

KCNK10 can be integrated to a K2P subfamily which includes K2P2.1 (TREK1) and K2P4.1 (TRAAK) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, low intracellular pH and mechanical stretch. KCNK10 mRNA expression has been detected in the brain (primarily in the cerebellum) and in peripheral tissues such as pancreas and kidney.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-KCNK10 (TREK-2) AntibodyExpression of TREK-2 in HEK-293 transfected cellsImmunocytochemical staining of HEK-293 K2P transfected cells using Anti-KCNK10 (TREK-2) Antibody (#APC-055). TREK-2 staining is specific for HEK-293 cells transfected with TREK-2.Adapted from Acosta, C. et al. (2014) J. Neurosci. 34, 1494. with permission of The Society for Neuroscience.

Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KCNK10. Anti-KCNK10 (TREK-2) Antibody (#APC-055) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNK10 channel from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat DRGs and plantar skin lysates (1:500).
    Acosta, C. et al. (2014) J. Neurosci. 34, 1494.
Immunohistochemistry citations
  1. Rat DRG sections (1:100).
    Liu, Z. et al. (2016) Mol. Pain 12, 1.
  2. Rat DRG sections (1:500).
    Acosta, C. et al. (2014) J. Neurosci. 34, 1494.
Immunocytochemistry citations
  1. HEK-293 transfected cells (1:500).
    Acosta, C. et al. (2014) J. Neurosci. 34, 1494.
More product citations
  1. de la Pena, E. et al. (2012) PLoS ONE 7, e52475.
  2. Lin, W. et al. (2004) J. Neurophysiol. 92, 2909.
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