Anti-Kir2.3 (KCNJ4) Antibody

Inward rectifier potassium channel 4, BIR11, IRK3, HIRK2, HRK1
    Cat #: APC-032
    Alternative Name Inward rectifier potassium channel 4, BIR11, IRK3, HIRK2, HRK1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EFGS HLDLE RMQAA TLPLD N, corresponding to amino acid residues 418-437 of rat Kir2.3 (Accession P52190). Intracellular, C-terminal part.
        Anti-Kir2.3 (KCNJ4) Antibody
    Accession (Uniprot) Number P52190
    Gene ID 116649
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; guinea pig - 19/20 amino acid residues identical; hamster, human, Xenopus - 18/20 amino acid residues identical.
    RRID AB_2040111.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-Kir2.3 (KCNJ4) Antibody
        Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:
        1,2. Anti-Kir2.3 (KCNJ4) Antibody (#APC-032), (1:200)
        3,4. Anti-Kir2.3 (KCNJ4) Antibody, preincubated with the control peptide antigen.
        Human heart lysate (1:200) (Voigt, N. et al. (2010) Circ. Arrhyth. Electrophysiol. 3, 472.).
      • Rat brain sections.
      • Canine ventricle and atrium myocytes (1:300) (Melnyk, P. et al. (2002) Am. J. Physiol. 283, H1123.).
    References
    • 1. Nichols, C.G. et al. (1996) Circ. Res. 78, 1.
    • 2. Melnyk, P. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 283, 1123.
      • Kir2.3 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

        The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers.

        Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.

        Kir2.3 is a member of the Kir2.x subfamily that includes four members (Kir2.1- Kir2.4) that are characterized by strong inward rectification and high constitutive activity.

        Kir2.3 is expressed in a variety of tissues including heart and brain. 2

        In the heart, Kir2.3 forms heteromers with Kir2.1 and underlay the IK1 current (at least in some species) that is responsible for setting the resting membrane potential, preventing membrane hyperpolarization due to Na+ pump activity, influencing propagation velocity, altering the electrical space constant, and promoting late phase repolarization.1,2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Kir2.3 (KCNJ4) AntibodyExpression of Kir2.1 and Kir2.3 channels in Lamina I pacemaker cells.Immunohistochemical staining of rat spinal cord sections using Lamina I pacemaker using Anti-Kir2.1 (KCNJ2) Antibody (#APC-026) and Anti-Kir2.3 (KCNJ4) Antibody (#APC-032). Confocal images of representative biocytin-filled pacemaker neurons (red) processed for Kir (green). Merged images demonstrate that immunoreactive puncta for Kir2.1 and Kir2.3 (yellow; inset) are localized to identified pacemaker neurons within lamina I of the neonatal spinal cord (right). Scale bars: 10 μm; inset, 2 μm.Adapted from Li, J. et al. (2013) with permission of The Society for Neuroscience.
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Kir2.3 channel. Anti-Kir2.3 (KCNJ4) Antibody (#APC-032) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir2.3 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Human heart lysate (1:200).
        Voigt, N. et al. (2010) Circ. Arrhyth. Electrophysiol. 3, 472.
      • Canine atrium and ventricle lysate (1:1000).
        Melnyk, P. et al. (2002) Am. J. Physiol. 283, H1123.
      • Rat dorsal root ganglia and spinal cord sections (1:200).
        Murata, Y. et al. (2016) Neurosci. Lett. 617, 59.
      • HEK 293 transfected cells (1:200).
        Murata, Y. et al. (2016) Neurosci. Lett. 617, 59.
      • Canine ventricle and atrium myocytes (1:300).
        Melnyk, P. et al. (2002) Am. J. Physiol. 283, 1123.
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