Anti-KV4.3 Antibody

Voltage-gated K+ channel subfamily D member 3, KCND3
    Cat #: APC-017
    Alternative Name Voltage-gated K+ channel subfamily D member 3, KCND3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)NEALELTGTPEEEHMGK, corresponding to amino acid residues 451-468 of human KV4.3 (Accession O60577). Intracellular, C-terminus.
        Anti-KV4.3 Antibody
    Accession (Uniprot) Number O60577
    Gene ID 3752
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, rabbit - identical; mouse - 16/17 amino acid residues identical.
    RRID AB_2040178.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
      • Anti-KV4.3 Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-KV4.3 Antibody (#APC-017), (1:200).
        2. Anti-KV4.3 Antibody, preincubated with the negative control antigen.
        Human heart lysate (1:250) (Zicha, S. et al. (2004) J. Physiol. 561, 735.).
      • Canine heart lysate (5 μg) (Doronin, S.V. et al. (2004) J. Biol. Chem. 279, 48231.).
      • Anti-KV4.3 Antibody
        Expression of KV4.3 in rat hippocampal CA1 region
        Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV4.3 Antibody (#APC-017), (1:400), followed by donkey anti-rabbit-Cy3 antibody (red). Staining appears in neurons of the CA1 hippocampal region (arrow). Nuclei are stained with DAPI (blue).
        Mouse colon lysate (1:100) (Amberg, G.C. et al. (2002) J. Physiol544.2, 403.).
      • HEK-293 transfected cells (1:50) (Hatano, N. et al. (2002) Pflugers Arch. 444, 80.).
    References
    • 1. Serodio, P. et al. (1994) J. Neurophysiol. 72,1516.
    • 2. Tsaur, M.L. et al. (1997) FEBS Lett. 400, 215.
    • 3. Guo, W. et al. (2002) Circ. Res. 90, 586.
    • 4. Diochot, S. et al. (1999) Br. J. Pharmacol. 126, 251.
      • KV4.3 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.1 and KV4.2.

        KV4.3 possesses the signature structure of the voltage-dependent K+ channels; six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.2.

        The KV4 channels are characterized by activation at subthreshold membrane potentials, rapid inactivation and fast recovery from inactivation compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents.1

        The biophysical properties of the KV4.3 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increases current densities and accelerates both the inactivation and the recovery time.

        KV4.3 is highly expressed in the brain where it has a key role in shaping the action potential and firing frequency of neurons. In the heart, together with KV4.2, it underlies the fast inactivating and recovering cardiac transient outward current Ito.2,3 The channel is also expressed in smooth muscle cells of several organs such as myometrium, lung and colon where its function has not been completely elucidated.

        Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.3 channels. Among these the most potent and selective are Phrixotoxin-1 (#STP-700) (28 nM) and Phrixotoxin-2 (#STP-710) (71 nM).4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-KV4.3 AntibodyExpression of KV4.3 increases in DA substantia-nigra neurons of α-synuclein mutant mice but not in DA neurons in the ventral tegmental area.Immunohistochemical staining of mouse brain sections using Anti-KV4.3 Antibody (#APC-017). A. Expression of KV4.3 increases in DA substantia-nigra neurons of α-synuclein mutant mice (lower right panel). B. Expression of KV4.3 does not increase in DA neurons of the ventral tegmental area in α-synuclein mutant mice (lower right panel).Adapted from Subramaniam, M. et al. (2014) with permission of the Society for Neuroscience.
    Last update: 14/01/2020

    Anti-KV4.3 Antibody (#APC-017) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV4.3 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Rat heart lysate.
        Argenziano, M. et al. (2017) J. Physiol. Sci. 217.
      • Rat heart lysate.
        Liu, X. et al. (2016) Cell. Physiol. Biochem. 39, 102.
      • Mouse heart lysate (1:5000).
        El Gebeily, G. et al. (2015) J. Mol. Cell. Cardiol. 86, 85.
      • Rat ventricular myocytes (1:200).
        Liu, W.J. et al. (2015) Am. J. Physiol. 309, H1288.
      • Rat cardiomyocyte lysate.
        Gomez-Hurtado, N. et al. (2013) Pflugers Arch. 466, 903.
      • Human heart lysate (1:250).
        Zicha, S. et al. (2004) J. Physiol. 561, 735.
      • Canine heart lysate (5 μg).
        Doronin, S.V. et al. (2004) J. Biol. Chem. 279, 48231.
      • Rat brain sections (1:500).
        Rizwan, A.P. et al. (2016) J. Neurosci. 36, 11196.
      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
      • Mouse embryo sections.
        Bye, C.R. et al. (2015) Proc. Natl. Acad. Sci. U.S.A. 112, E1946.
      • Mouse brain sections.
        Subramaniam, M. et al. (2014) J. Neurosci. 34, 13586.
      • Mouse colon sections (1:100).
        Amberg, G.C. et al. (2002) J. Physiol. 544.2, 403.
      • HEK 293 transfected cells (1:50).
        Hatano, N. et al. (2002) Pflugers Arch. 444, 80.
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