Anti-KV4.1 (KCND1) Antibody

Voltage-gated potassium channel subfamily D member 1
    Cat #: APC-119
    Alternative Name Voltage-gated potassium channel subfamily D member 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KRRAIRLANSTAS, corresponding to amino acid residues 538-550 of human KCND1 (Accession Q9NSA2). Intracellular, C-terminal domain.
        Anti-KV4.1 (KCND1) Antibody
    Accession (Uniprot) Number Q9NSA2
    Gene ID 3750
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 12/13 amino acid residues identical.
    RRID AB_2040174.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-KV4.1 (KCND1) Antibody
        Western blot analysis of rat brain lysate:
        1. Anti-KV4.1 (KCND1) Antibody (#APC-119), (1:400).
        2. Anti- KV4.1 (KCND1) Antibody, preincubated with the negative control antigen.
        Anti-KV4.1 (KCND1) Antibody
        Western blot analysis of human neuroblastoma cell line SH-SY5Y (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
        1,2. Anti-KV4.1 (KCND1) Antibody (#APC-119), (1:400).
        3,4. Anti-KV4.1 (KCND1) Antibody, preincubated with the negative control antigen.
      • Human breast cancer tissue (1:200) (Jang, S.H. et al. (2009) Biochem. Biophys. Res. Commun. 384, 180.).
      • Mouse dissociated preoptic neurons (Sethi, J. et al. (2011) PLoS ONE 6, e29134.).
    References
      • KV4.1 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.2 and KV4.3.

        KV4.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.2.

        The KV4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of KV4.1 and KV4.2 subunits. The biophysical properties of the KV4.1 subunit can be modified by its association with auxiliary b subunits such as KChIP1 that increase KV4.1 current densities and accelerates both the inactivation and the recovery time.

        KV4.1 is highly expressed in the brain but is also expressed in peripheral tissues such as the colon, heart and lung.

        The pharmacology of KV4.1 has been less well studied than that of the other KV4 members. KV4.1 is sensitive to the classical K+ channel blockers, 4-aminopyridine and tetraethylammonium. Recently, HmTx1, a peptide blocker derived from the venom of the African tarantula Heteroscodra maculata, has been shown to be a potent KV4.1 inhibitor.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of human KV4.1. The Anti-KV4.1 (KCND1) Antibody (#APC-119) can be used for western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV4.1 from rat, human, and mouse samples.

    For research purposes only, not for human use