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Anti-MAGI2/AIP1 Antibody

Membrane associated guanylate kinase, WW and PDZ domain containing 2, Activin receptor-interacting protein 1, Acvrip1, Atrophin-1-interacting protein 1
Cat #: APZ-052
Alternative Name Membrane associated guanylate kinase, WW and PDZ domain containing 2, Activin receptor-interacting protein 1, Acvrip1, Atrophin-1-interacting protein 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ENSYRSEVKARQDVK, corresponding to amino acid residues 1066-1080 of mouse MAGI2 (Accession Q9WVQ1). Intracellular, C-terminus.
Accession (Uniprot) Number Q9WVQ1
Gene ID 50791
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-MAGI2/AIP1 Antibody (#APZ-052), (1:400).
    2. Anti-MAGI2/AIP1 Antibody, preincubated with MAGI2/AIP1 Blocking Peptide (#BLP-PZ052).
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-MAGI2/AIP1 Antibody (#APZ-052), (1:400).
    2. Anti-MAGI2/AIP1 Antibody, preincubated with MAGI2/AIP1 Blocking Peptide (#BLP-PZ052).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-MAGI2/AIP1 Antibody (#APZ-052), (1:200).
    2. Anti-MAGI2/AIP1 Antibody, preincubated with MAGI2/AIP1 Blocking Peptide (#BLP-PZ052).
References
  1. Nagashima, S. et al. (2015) J. Biochem. 157, 177.
  2. Empitu, M.A. et al. (2018) Am. J. Physiol. Renal Physiol. 315, F1336.
  3. David, S.N. et al. (2018) Prostate 78, 616.
  4. Koide, T. et al. (2012) PLoS One 7, e36836.
  5. Wu, X. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 4233-8.
Scientific background

MAGI2 is a member in the Membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains (MAGI) family, and includes two additional family members, MAGI1 and MAGI31.

MAGI proteins are essential for efficient cell signaling as they function as scaffolds for assembling cell adhesion molecules, receptors and signaling molecules and linking them to the cytoskeleton2.

MAGI2 has a guanylate kinase, 2 WW, and 6 PDZ domains (PDZ0-PDZ5). It interacts with cytoskeleton-associated proteins: guanylate-kinase-associated protein (GKAP), Postsynaptic density-95 (PSD-95)/synapse-associated protein 90 (SAP90), Stargazin/transmembrane AMPAR regulatory proteins (TARPs). MAGI2 also interacts with N-methyl-D-aspartate (NMDA) receptors, δ2 Glutamate receptor, β1AR, VPAC1, Brain-specific angiogenesis inhibitor-1, Activin Type II receptors, Neuroligins, β-Dystroglycan, Dendrite arborization and synapse maturation 1 (Dasm1)/IgSF9b, Nectins and Nephrin receptors and cell adhesion proteins, respectively1,3.

MAGI2 expression is almost exclusively in brain, deletion of MAGI2 causes infantile spasm and increased cognitive impairment in schizophrenia patients4.

In non-neuronal tissues, MAGI2 enhances the ability of PTEN to suppress Akt activation in the PI3-kinase/Akt pathway. PTEN binds to MAGI2 through the PDZ domains, thus enhancing PTEN stability and improves the efficiency of PTEN signalling, allowing for efficient tumor suppression by PTEN1,5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-MAGI2/AIP1 Antibody (#APZ-052) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize AIP1 from human, rat, and mouse samples.

For research purposes only, not for human use
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