Anti-mGluR4 (extracellular) Antibody

Metabotropic Glutamate Receptor 4, GRM4, mGlu4
    Cat #: AGC-014
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide KPKGHPHMNSIR(C), corresponding to amino acid residues 33-44 of rat mGluR4 (Accession P31423). Extracellular, N-terminus.
    Accession (Uniprot) Number P31423
    Gene ID 24417
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1, 2. Anti-mGluR4 (extracellular) Antibody (#AGC-014), (1:200).
    3, 4. Anti-mGluR4 (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of mGluR4 in rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells using Anti-mGluR4 (extracellular) Antibody (#AGC-014), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B. Nuclei are stained with Hoechst 33342 (blue).
    References
    1. Kim, C.H. et al. (2008) J. Neurosci. Res. 86, 1.
    2. Niswender, C.M. and Conn, P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50, 295.
    3. Chaudhari, N. et al. (2000) Nat. Neurosci. 3, 113.
    4. Chaudhari, N. et al. (2009) Am. J. Clin. Nutr. 90, 738S.
    5. Pekhletski, R. et al. (1996) J. Neurosci. 16, 6364.
    6. Conn, P.J. et al. (2005) Nat. Rev. Neurosci. 6, 787.
    Scientific background

    Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.

    The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2.

    mGluR4 is predominantly expressed presynaptically in neurons and in the cerebellum. A splice variant of the protein is expressed in taste buds. This variant lacks a large portion of the N-terminus and is normally referred to taste mGluR43,4. It is responsible (along with taste mGluR1) for mediating the taste of monosodium glutamate (or unami)2,4.

    mGluR4 knock out mice display impaired cerebellar synaptic plasticity as well as some learning disabilities5. In addition, this receptor has emerged as a target for the treatment of Parkinson’s disease6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR4. Anti-mGluR4 (extracellular) Antibody (#AGC-014) can be used in western blot and live cell imaging applications. It has been designed to recognize mGluR4 from rat, mouse and human samples.

    For research purposes only, not for human use