Anti-NRG1 Type III (extracellular) Antibody

Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF

Cat #: ANR-113
Alternative Name Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DKIFEYDSPTHLD, corresponding to amino acid residues 101-113 of human Neuregulin-1 Type III (Accession Q02297-10 and Q02297). Extracellular.
Accession (Uniprot) Number Q02297
Gene ID 3084
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – identical.
RRID AB_2756704.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity This antibody will recognize Neuregulin-1 Type III. It will not recognize Neuregulin-1 Type I, Type II and Type IV.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysate:
    Western blot analysis of rat dorsal root ganglion lysate:
    1. Anti-NRG1 Type III (extracellular) Antibody (#ANR-113), (1:200).
    2. Anti-NRG1 Type III (extracellular) Antibody, preincubated with NRG1 Type III (extracellular) Blocking Peptide (#BLP-NR113).
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1,2. Anti-NRG1 Type III (extracellular) Antibody (#ANR-113), (1:200).
    3,4. Anti-NRG1 Type III (extracellular) Antibody, preincubated with NRG1 Type III (extracellular) Blocking Peptide (#BLP-NR113).
Immunohistochemistry
  • Mouse brain sections (Gatius, A. et al. (2020) Front. Cell. Neurosci. 13, 582.).
References
  1. Huang, L.L. et al. (2015) Neural. Regen. Res. 10, 65.
  2. Pan, P. et al. (2013) Acta. Neuropathol. Commun. 1, 39.
  3. Jiang, L. et al. (2013) J. Neurosci. 33, 9655.
Scientific background

Neuregulin-1 (NRG1) is a member of a subfamily of the epidermal growth factor family and plays an important role in the development of the peripheral nervous system. Through alternative RNA splicing and promoter usage, the NRG1 gene encodes an entire family of more than 15 transmembrane and secreted isoforms. Neuregulin-1 acts as an important regulator in the survival of Schwann cell precursors, Schwann cell proliferation, motility, axon ensheathment and myelination.

Neuregulin-1 can be divided into different according to the N-terminal sequence. Neuregulin-1 Type III, a transmembrane protein, contains an epidermal growth factor-like domain, which alone is adequate to bind and activate ErbB receptor tyrosine kinases. Type III also contains a cysteine-rich domain called “cysteine-rich domain-neuregulins,” that requires a proteolytic cleavage for full activity1,2.

Knockout studies show that type III is the key isoform required for schwann cell generation. These studies also confirmed that type III isoform is necessary for promoting myelination and to regulate myelin thickness1,2

Several different studies also showed that NRG1 Type III isoform is also an important regulator of cholinergic signaling. Neuregulin-1 Type III regulates the expression of AChR genes and targeting of nAChRs to the neuronal cell surface. This isoform in particular is a critical regulator of presynaptic targeting of α7-containing nAChRs3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-NRG1 Type III (extracellular) Antibody (#ANR-113) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NRG1 Type III from rat, human and mouse samples. The antibody is specific for NRG1 Type III and does not recognize other Neuregulin-1 isoforms.

For research purposes only, not for human use
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