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Anti-NPY2R Antibody

Neuropeptide Y2 receptor, Neuropeptide Y receptor type 2, NPY Y2 receptor

Cat #: ANR-022
Alternative Name Neuropeptide Y2 receptor, Neuropeptide Y receptor type 2, NPY Y2 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide CEQRLDAIHSEVSMTFKAK, corresponding to amino acid residues 346-364 of mouse NPY2R (Accession P97295). Intracellular, C-terminus.
Accession (Uniprot) Number P97295
Gene ID 18167
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human - 18/19 amino acid residues identical.
RRID AB_2040032.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat hippocampus and rat whole brain lysates:
    Western blot analysis of rat hippocampus and rat whole brain lysates:
    1,2. Anti-NPY2R Antibody (#ANR-022), (1:200).
    3,4. Anti-NPY2R Antibody, preincubated with NPY2R Blocking Peptide (#BLP-NR022).
Immunohistochemistry
  • Expression of neuropeptide Y2 receptor in rat cerebellum
    Expression of neuropeptide Yreceptor in rat cerebellum
    Immunohistochemical staining of frozen section of rat cerebellum using Anti-NPY2R Antibody (#ANR-022), (1:100). NPY2R (red) is detected in Purkinje cells. B. Nuclei were stained using DAPI as the counterstain. C. Merged image of A and B.
Immunocytochemistry
  • Expression of neuropeptide Y2 receptor in rat dorsal root ganglion cells
    Expression of neuropeptide Yreceptor in rat dorsal root ganglion cells
    Immunocytochemical staining of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY2R Antibody (#ANR-022), (1:100) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.
    Magnification:
    A-C: x20
    E-F: x100
References
  1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357.
  2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426.
  3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391.
  4. Bournat, J.C. and Allen, J.M. (2001) Brain Res. Mol. Brain Res. 90, 149.
  5. Wheway, J. et al. (2005) J. Exp. Med. 202, 1527.
  6. Silva, A.P. et al. (2006) FASEB J. 21, 671.
  7. Batterham, R.L. et al. (2003) N. Engl. J. Med. 349, 941.
Scientific background

Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2

NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2

The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2

NPY2R is primarily expressed in neurons as a presynaptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney.4 NPY2R is involved in inhibition of neurotransmitter release mediated by NPY.Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor.6 Involvement of NPY2R in feeding behavior was also reported. PYY(3–36), an NPY2R agonist, acts to inhibit feeding via NPY2R.7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse neuropeptide Y2 receptor (NPY2R). Anti-NPY2R Antibody (#ANR-022) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY2R from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Rat retinal sections (1:2000).
    Martins, J. et al. (2015) ASN. Neuro. 7, 4.
Immunocytochemistry citations
  1. Rat retinal ganglion cells (RGC), (1:500).
    Martins, J. et al. (2015) ASN. Neuro. 7, 4.
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