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Anti-NPY4R Antibody

Neuropeptide Y4 receptor, Neuropeptide Y receptor type 4, Pancreatic polypeptide receptor 1, PPYR1

Cat #: ANR-024
Alternative Name Neuropeptide Y4 receptor, Neuropeptide Y receptor type 4, Pancreatic polypeptide receptor 1, PPYR1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R (Accession Q63447). Intracellular, C-terminus.
Accession (Uniprot) Number Q63447
Gene ID 29471
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/15 amino acid residues identical.
RRID AB_2040034.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain membrane (lanes 1 and 4), cortex (lanes 2 and 5), and hippocampus (lanes 3 and 6) lysates:
    Western blot analysis of rat brain membrane (lanes 1 and 4), cortex (lanes 2 and 5), and hippocampus (lanes 3 and 6) lysates:
    1,2,3. Anti-NPY4R Antibody (#ANR-024), (1:200).
    4,5,6. Anti-NPY4R Antibody, preincubated with NPY4R Blocking Peptide (#BLP-NR024).
Immunohistochemistry
  • Rat retinal sections (1:200) (Martins, J. et al. (2015) ASN. Neuro. 7, 4.).
Immunocytochemistry
  • Expression of neuropeptide Y4 receptor in rat DRG
    Expression of neuropeptide Y4 receptor in rat DRG
    Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY4R Antibody (#ANR-024), (1:100), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of A and B. F. Merged images of D and E. 
    Magnification:
    A-C: x20.
    E-F: x100.
References
  1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357.
  2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426.
  3. Sun, L. et al. (1999) J. Neurophysiol. 81, 1391.
  4. Larhammar, D. et al. (2001) Peptides 22, 295.
  5. Voisin, T. et al. (2000) J. Pharmacol. Exp. Ther. 292, 638.
Scientific background

Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, along with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in the regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2

NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2

The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2

NPY4R preferentially binds PP, while NPY1R, NPY2R, and NPY5R bind both NPY and PP.

NPY1R, NPY2R, and NPY5R share a low degree of identity (27–31% overall). NPY4R is more similar to NPY1R, displaying an overall identity of 43–53%.4

Human NPY4R (also named pancreatic polypeptide receptor 1) mRNA is highly expressed in the small and large intestines and the prostate. Tissue distribution studies in human and mouse suggest potential roles for NPY4R in the gastrointestinal tract, heart, and prostate, as well as in neural and endocrine signaling. Only low levels of NPY4R expression in various areas of brain were displayed.5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat neuropeptide Y4 receptor (NPY4R). Anti-NPY4R Antibody (#ANR-024) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY4R from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Rat retinal sections (1:200).
    Martins, J. et al. (2015) ASN. Neuro. 7, 4.
Immunocytochemistry citations
  1. Rat retinal ganglion cells (RGC), (1:25).
    Martins, J. et al. (2015) ASN. Neuro. 7, 4.
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