Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody

nAChR β3, Neuronal acetylcholine receptor subunit beta-3
    Cat #: ANC-013
    Alternative Name nAChR β3, Neuronal acetylcholine receptor subunit beta-3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)NAKGMKGNRREG, corresponding to amino acid residues 211-222 of rat nAChRβ3 (Accession P12391). Extracellular, N-terminus.
        Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody
    Accession (Uniprot) Number P12391
    Gene ID 171131
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 11/12 amino acid residues identical.
    RRID AB_2341001.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, lci, wb
    May also work in: ifc*, ih*, ip*
      • Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:400).
        2. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody
        Expression of nAChR β3 in live intact rat PC12 cells
        Cell surface detection of nAChR β3 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge image of A and B.
    References
      • Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and having longer-term effects on gene expression and cellular connections1.

        nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits. To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1. The β3 subunit cannot form functional receptors when expressed alone. It only forms operational channels when coexpressed with other functional subunit combinations, and is thus commonly termed ‘orphan’ or ‘auxiliary’ subunits3.

        β3 nAChR subunit mRNA is highly expressed in dopaminergic neurons of the substantia nigra and ventral tegmental area4.

        α6/β3 nAChRs have been implicated in dopaminergic neurotransmission, nicotine dependence, anxiety, and other important neurophysiological processes5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat nAChR β3. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013) can be used in western blot and live cell imaging applications. It has been designed to recognize nAChRβ3 from mouse, rat and human samples.

    For research purposes only, not for human use