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2. Anti-Nicotinic Acetylcholine Receptor β3 (extracellular) Antibody, preincubated with the control peptide antigen.
Nicotinic Acetylcholine Receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and having longer-term effects on gene expression and cellular connections1.
nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits. To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1. The β3 subunit cannot form functional receptors when expressed alone. It only forms operational channels when coexpressed with other functional subunit combinations, and is thus commonly termed ‘orphan’ or ‘auxiliary’ subunits3.
β3 nAChR subunit mRNA is highly expressed in dopaminergic neurons of the substantia nigra and ventral tegmental area4.
α6/β3 nAChRs have been implicated in dopaminergic neurotransmission, nicotine dependence, anxiety, and other important neurophysiological processes5.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Nicotinic Acetylcholine Receptor β3 (nAChRβ3). Anti-Nicotinic Acetylcholine Receptor β3 (extracellular) Antibody (#ANC-013) can be used in western blot and live cell imaging applications. It has been designed to recognize nAChRβ3 from mouse, rat and human samples.
- Anti-Nicotinic Acetylcholine Receptor β1 (extracellular) Antibody (#ANC-011)
- Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012)
- Anti-Nicotinic Acetylcholine Receptor β4 (extracellular) Antibody (#ANC-014)
- Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001)
- Anti-Nicotinic Acetylcholine Receptor α7 (extracellular) Antibody (#ANC-007)