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Anti-NPY1R (extracellular)-FITC Antibody

Neuropeptide Y receptor type 1, NPYR, NPYY1

Cat #: ANR-027-F
Alternative Name Neuropeptide Y receptor type 1, NPYR, NPYY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CFDKFPSDSHRLSY, corresponding to amino acid residues 197-210 of mouse NPY1R (Accession Q04573). 2nd extracellular loop.
Accession (Uniprot) Number Q04573
Gene ID 18166
Homology Rat – identical; human – 13/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-027), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of NPY1R by direct flow cytometry in live intact mouse J774 macrophage cell line:
    Cell surface detection of NPY1R by direct flow cytometry in live intact mouse J774 macrophage cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-NPY1R (extracellular)-FITC Antibody (#ANR-027-F), (2.5µg).
  • Cell surface detection of NPY1R by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of NPY1R by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-NPY1R (extracellular)-FITC Antibody (#ANR-027-F), (5µg).
References
  1. Yang, Z. et al. (2018) Nature 556, 520.
  2. Comeras, L.B. et al. (2019) Ann. N.Y. Acad. Sci. 1455, 59.
  3. Fetissov, S.O. et al. (2004) Neuropeptides 38, 175.
  4. Dumont, Y. et al. (1998) J. Comp. Neurol. 402, 372.
  5. Holzer, P. et al. (2012) Neuropeptides 46, 261.
Scientific background

Neuropeptide Y1 receptor (NPY1R) is one out of four receptors (NPY1RNPY2RNPY4R and NPY5R) that bind neuropeptide Y (NPY), an abundant neuropeptide in the mammalian central and peripheral nervous systems.

NPY1R is part of the β-branch of class A G-protein coupled receptors and likewise, has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1,2.

NPY belongs to a family of gut-brain peptides that demonstrate both hormonal functions by paracrine and endocrine release in the gastrointestinal tract and a neurotransmitter functions in sympathetic ganglia and the CNS. It is highly expressed throughout the brain with particularly high expression in limbic brain areas, such as the hippocampus, amygdala, and hypothalamus3,4. NPY modulates multiple physiological and pathophysiological processes: it increases food intake and reduces fear and anxiety by activating NPY1R1,2.

Because of the multiple functions of NPY, NPY1R has great importance in the development and progression of both metabolic and neurological diseases such as diabetes, obesity, breast cancer and bone loss and indeed a number of peptides and small-molecule compounds have been characterized as NPY1R antagonists and have shown clinical potential in the treatment of these pathological states5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 20/10/2021

Anti-NPY1R (extracellular) Antibody (#ANR-027) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NPY1R from mouse, rat and human samples.

Anti-NPY1R (extracellular)-FITC Antibody (#ANR-027-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NPY1R from mouse, rat and human samples.

For research purposes only, not for human use
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