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Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody

Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1

Cat #: AOR-012
Alternative Name Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)NGSVGSEDQQLEPAHIS, corresponding to amino acid residues 39-55 of rat κ-Opioid receptor (Accession P34975). Extracellular, N-terminus.
Accession (Uniprot) Number P34975
Gene ID 29335
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse -16/17 amino acid residues identical; human - 15/17 amino acid residues identical.
RRID AB_2756739.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain (lanes 1 and 3), rat brain (lanes 2 and 4) and human brain neuroblastoma (SH-SY5Y) cell (lanes 5 and 6) lysates:
    Western blot analysis of mouse brain (lanes 1 and 3), rat brain (lanes 2 and 4) and human brain neuroblastoma (SH-SY5Y) cell (lanes 5 and 6) lysates:
    1,2,5. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (#AOR-012), (1:200).
    3,4,6. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody, preincubated with κ-Opioid Receptor/OPRK1 (extracellular) Blocking Peptide (#BLP-OR012).
References
  1. Pan, L. et al. (2005) Neuroscience 133, 209.
  2. IUPHAR
  3. Matthes, H.W. et al. (1998) J. Neurosci. 18, 7285.
  4. Di Chiara, G. and North, R.A. (1992) Trends Pharmacol. Sci. 13, 185.
  5. Schwarzer, C. (2009) Pharmacol. Ther. 123, 353.
Scientific background

Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor1. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors2.

The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins1.

All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-opioid receptor, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level3. The opioid receptors also mediate the mood-altering properties of opioids4.

κ-Opioid Receptor is activated by the endogenous peptide dynorphin. Malfunction of their signaling may be involved in epilepsy, addiction, depression, schizophrenia, and chronic pain5.

κ-Opioid Receptor is localized to a number of regions in the brain, as well as the spinal cord, the enteric nervous system and the digestive system5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat κ-opioid receptor. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (#AOR-012) can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. The antibody was designed to recognize κ-opioid receptor from mouse, rat and human samples.

For research purposes only, not for human use
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