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Anti-RAMP2 (extracellular) Antibody

Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
Cat #: ARR-022
Alternative Name Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)HPTEDSLLSKGKMED, corresponding to amino acid residues 54-68 of rat RAMP2 (Accession Q9JHJ1). Extracellular, N-terminus.
Accession (Uniprot) Number Q9JHJ1
Gene ID 58966
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 14/15 amino acid residues identical.
RRID AB_2756789.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat lung (lanes 1 and 4, 1:200), mouse heart (lanes 2 and 5, 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6, 1:400) lysates:
    Western blot analysis of rat lung (lanes 1 and 4, 1:200), mouse heart (lanes 2 and 5, 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6, 1:400) lysates:
    1-3. Anti-RAMP2 (extracellular) Antibody (#ARR-022).
    4-6. Anti-RAMP2 (extracellular) Antibody, preincubated with RAMP2 (extracellular) Blocking Peptide (#BLP-RR022).
  • Western blot analysis of rat spleen (lanes 1 and 3) and cortex (lanes 2 and 4):
    Western blot analysis of rat spleen (lanes 1 and 3) and cortex (lanes 2 and 4):
    1,2. Anti-RAMP2 (extracellular) Antibody (#ARR-022), (1:200).
    3,4. Anti-RAMP2 (extracellular) Antibody, preincubated with RAMP2 (extracellular) Blocking Peptide (#BLP-RR022).
Indirect flow cytometry
  • Cell surface detection of RAMP2 in live intact mouse J774 macrophage cells:
    Cell surface detection of RAMP2 in live intact mouse J774 macrophage cells:
    ___ Cells + goat anti-rabbit IgG-FITC antibody
    ___ Cells + Anti-RAMP2 (extracellular) Antibody (#ARR-022), (5 μg/0.5x106 cells).
References
  1. Cegla, J. et al. (2017) Endocrinology 158, 2680.
  2. Kadmiel, M. et al. (2017) PLoS One 12, e0181597.
  3. Kechele, D.O. et al. (2016) Hypertension 68, 667.
Scientific background

RAMP2, also known as receptor activity modifying protein 2, is a single-pass transmembrane protein with extracellular N-terminus and intracellular C-terminal tail. RAMP2 heterodimerizes with several G-protein coupled receptors (GPCRs) and alters their function and life cycle. RAMPs were first identified for the receptors association with calcitonin receptor-like receptor (Calcrl, CLR) as the canonical receptor for the endocrine peptide adrenomedullin (Adm, AM). In addition, RAMP2 also binds to other class B GPCRs: calcitonin receptor (Ctr), corticotrophin releasing hormone receptor 1 (Crhr1), glucagon receptor (Gcgr), parathyroid hormone receptor 1 (Pth1r), and vasoactive intestinal polypeptide receptor types 1 and 2 (VPAC1 and VPAC2). RAMPs have an important role in receptor trafficking, including translocation from the endoplasmic reticulum to the Golgi, internalization, and recycling of the receptors1,2.

The mammalian RAMP family includes three members, each encoded by single genes: RAMP1, RAMP2, and RAMP3. Studies have shown that Ramp2 null mice are embryonic lethal, with cardiovascular developmental defects in the heart, blood, and lymphatic vasculatures3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-RAMP2 (extracellular) Antibody (#ARR-022) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize RAMP2 from mouse, and rat samples. It will not recognize RAMP2 from human samples.

For research purposes only, not for human use
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