Anti-RAMP2 (extracellular) Antibody

Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
    Cat #: ARR-022
    Alternative Name Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)HPTEDSLLSKGKMED, corresponding to amino acid residues 54-68 of rat RAMP2 (Accession Q9JHJ1). Extracellular, N-terminus.
        Anti-RAMP2 (extracellular) Antibody
    Accession (Uniprot) Number Q9JHJ1
    Gene ID 58966
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical.
    RRID AB_2756789.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for use with human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
      • Anti-RAMP2 (extracellular) Antibody
        Western blot analysis of rat lung (lanes 1 and 4, 1:200), mouse heart (lanes 2 and 5, 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6, 1:400) lysates:
        1-3. Anti-RAMP2 (extracellular) Antibody (#ARR-022).
        4-6. Anti-RAMP2 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-RAMP2 (extracellular) Antibody
        Western blot analysis of rat spleen (lanes 1 and 3) and cortex (lanes 2 and 4):
        1,2. Anti-RAMP2 (extracellular) Antibody (#ARR-022), (1:200).
        3,4. Anti-RAMP2 (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-RAMP2 (extracellular) Antibody
        Cell surface detection of RAMP2 in live intact mouse J774 macrophage cells:
        ___ Cells + goat anti-rabbit IgG-FITC antibody
        ___ Cells + Anti-RAMP2 (extracellular) Antibody (#ARR-022), (5 μg/0.5x106 cells).
    References
      • RAMP2, also known as receptor activity modifying protein 2, is a single-pass transmembrane protein with extracellular N-terminus and intracellular C-terminal tail. RAMP2 heterodimerizes with several G-protein coupled receptors (GPCRs) and alters their function and life cycle. RAMPs were first identified for the receptors association with calcitonin receptor-like receptor (Calcrl, CLR) as the canonical receptor for the endocrine peptide adrenomedullin (Adm, AM). In addition, RAMP2 also binds to other class B GPCRs: calcitonin receptor (Ctr), corticotrophin releasing hormone receptor 1 (Crhr1), glucagon receptor (Gcgr), parathyroid hormone receptor 1 (Pth1r), and vasoactive intestinal polypeptide receptor types 1 and 2 (VPAC1 and VPAC2). RAMPs have an important role in receptor trafficking, including translocation from the endoplasmic reticulum to the Golgi, internalization, and recycling of the receptors1,2.

        The mammalian RAMP family includes three members, each encoded by single genes: RAMP1, RAMP2, and RAMP3. Studies have shown that Ramp2 null mice are embryonic lethal, with cardiovascular developmental defects in the heart, blood, and lymphatic vasculatures3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-RAMP2 (extracellular) Antibody (#ARR-022) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize RAMP2 from mouse, and rat samples. It will not recognize RAMP2 from human samples.

    For research purposes only, not for human use