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Anti-Ret Receptor (extracellular)-FITC Antibody

Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51

Cat #: ANT-025-F
Alternative Name Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret (Accession P07949). Extracellular, N-terminus.
Accession (Uniprot) Number P07949
Gene ID 5979
Homology Mouse - identical; rat - 12/13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-025), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of Ret receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of Ret receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Ret receptor (extracellular)-FITC Antibody (#ANT-025-F), (2.5µg).
  • Cell surface detection of Ret receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Ret receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Ret receptor (extracellular)-FITC Antibody (#ANT-025-F), (5µg).
References
  1. Durbec, P. et al. (1996) Nature 381, 789.
  2. Cabrera J.R. et al. (2011) J. Biol. Chem. 286, 14628.
  3. Anders, J. et al. (2001) J. Biol. Chem. 276, 35808.
  4. Runeberg-Roos, P. and Saarma, M. (2007) Ann. Med. 39, 572.
  5. Knowles, P.P. et al. (2006) J. Biol. Chem. 281, 33577.
Scientific background

The RET proto-oncogene on human chromosome 10q11.2, encodes a receptor tyrosine kinase (RTK) activated by members of the glial cell line-derived neurotrophic factor (GDNF) ligand family (GDNF, neurturin, artemin, and persephin) in conjunction with a ligand-specific coreceptor (GFRα1-4)1. RET belongs to the cadherin superfamily, and it has been suggested that the ret gene is the result of a recombination between a gene encoding a tyrosine kinase receptor and a gene encoding an ancestral cadherin at an early stage of evolution2. The extracellular domain of RET comprises, as observed for other tyrosine kinase receptors, a cysteine-rich domain, but also four cadherin-like domains3.

RET signaling is crucial for the development of the enteric nervous system. RET also regulates the development of sympathetic, parasympathetic, motor, and sensory neurons, and is necessary for the postnatal maintenance of dopaminergic neurons. Outside the nervous system, RET is crucial for development of the kidney and plays a key role in spermatogenesis4.

RET has attracted considerable clinical interest because of the range of mutations found in diverse conditions that include Hirschsprung disease and a variety of cancers involving the thyroid gland. RET-related cancers with thyroid involvement include sporadic and familial medullary thyroid carcinoma (MTC), multiple endocrine neoplasia 2 (MEN2) syndromes MEN2A and MEN2B, and papillary thyroid carcinoma (PTC)5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 18/07/2022

Anti-Ret Receptor (extracellular) Antibody (#ANT-025) is a highly specific antibody directed against an extracellular epitope of the human Ret receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize Ret receptor from mouse, rat and human samples.

Anti-Ret Receptor (extracellular)-FITC Antibody (#ANT-025-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize Ret receptor from mouse, rat and human samples.

For research purposes only, not for human use
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