Anti-Semaphorin-3F Antibody

Semaphorin IV, SEMA3F
  • New
Cat #: ASR-056
Alternative Name Semaphorin IV, SEMA3F
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RERSAEAPQSPAVYAR, corresponding to amino acid residues 280 - 295 of human Semaphorin-3F (Accession Q13275). Secreted.
    • Anti-Semaphorin-3F Antibody
    Accession (Uniprot) Number Q13275
    Gene ID 6405
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
    Western blot
    • Anti-Semaphorin-3F Antibody
      Western blot analysis of rat brain membranes (lanes 1 and 5), rat lung membranes (lanes 2 and 6), mouse heart membranes (lanes 3 and 7) and rat dorsal root ganglion lysate (lanes 4 and 8):
      1-4. Anti-Semaphorin-3F Antibody (#ASR-056), (1:400).
      5-8. Anti-Semaphorin-3F Antibody, preincubated with the negative control antigen.
    • Anti-Semaphorin-3F Antibody
      Western blot analysis of human HeLa cervix adenocarcinoma cell line lysate (lanes 1 and 3) and human PC-3 prostate adenocarcinoma cell line lysate (lanes 2 and 4):
      1, 2. Anti-Semaphorin-3F Antibody (#ASR-056), (1:400).
      3, 4. Anti-Semaphorin-3F Antibody, preincubated with the negative control antigen.
    References
    1. Neufeld, G. et al. (2005) Front. Biosci. 10, 751.
    2. Xiang, R.H. et al. (1996) Genomics 32, 39
    3. Kolodkin, A.L. et al. (1993) Cell 75, 1389.
    Scientific background

    Semaphorins are a large family of proteins with over 30 members, which are further divided in subgroups1. They are involved in signaling and are implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration2. They are highly expressed in mammary gland, kidney, fetal brain, and lung and expressed at lower levels in heart and liver tissues2.

    All semaphorin members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues3. The semaphorin 3 family have a basic domain at the C-terminus. They bind neuropilins which subsequently activate signaling through associated plexins. Semaphorin-3B and semaphorin-3F function additionally as potent inhibitors of tumor development in small cell lung carcinoma. Recent evidence indicates that these semaphorins modulate the adhesive and migratory properties of responsive malignant cells. Semaphorin-3F was also functions as an inhibitor of angiogenesis, contributing significantly to its anti-tumorigenic properties1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 24/06/2020

    Anti-Semaphorin-3F Antibody (#ASR-056) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SEMA3F from mouse, rat, and human samples.

    For research purposes only, not for human use
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