Anti-Neuropilin-2 (NRP2) (extracellular) Antibody

Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
    Cat #: ANR-062
    Alternative Name Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)REDQGGEWKHGR, corresponding to amino acid residues 747-758 of human neuropilin-2 (Accession O60462). Extracellular, N-terminus.
    • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
    Accession (Uniprot) Number O60462
    Gene ID 8828
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse – 11/12 amino acid residues identical.
    RRID AB_2756693.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
      Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):
      1,2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).
      3,4. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
      Western blot analysis of human U-97 MG glioma cell line lysate:
      1. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).
      2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
      Expression of Neuropilin-2 in mouse substantia nigra pars compacta
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:400). A. NRP2 staining (green) reveals several neuronal profiles of NRP2 positive neurons that are distributed along the substantia nigra pars compacta. B. The same section stained for Calbindin-D28K (red) shows positive neurons along the substantia nigra pars compacta. C. Merge of the two images reveals co-localization in several neurons (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).
    Indirect flow cytometry
    • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
      Cell surface detection of Neuropilin-2 in live intact human THP-1 acute monocytic leukemia cell line:
      ___ Unstained cells + goat anti-rabbit-AlexaFluor-647 secondary antibody.
      ___ Cells + Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:15) + goat anti-rabbit-AlexaFluor-647 secondary antibody.
    References
    1. Wild, J.R. et al. (2012) Int. J. Exp. Pathol. 93, 81.
    2. Prud'homme, G.J. and Glinka, Y. (2012) Oncotarget 3, 921.
    3. Bagri, A. et al. (2009) Clin. Cancer Res. 15, 1860.
    Scientific background

    The neuropilins (NRPs) are single-pass multifunctional transmembrane glycoproteins that play an important role in cell development, immunity and cancer, and physiological and pathological angiogenesis. They were first discovered as regulators of nervous system development, acting as semaphorin co-receptors with plexins, as well as enhancers of cellular responses to members of the vascular endothelial growth factor (VEGF) family.

    The NRP family comprises two members: Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2), which are usually expressed as homodimers but can also appear as heterodimers. NRP2 contains a large extracellular N-terminal which consists of five domains, a short transmembrane domain and a small cytoplasmic domain1-3

    NRP2 is expressed in a wide variety of cells including endothelial cells, neurons, pancreatic islet cells, hepatocytes, melanocytes and osteoblasts. NRP2 has been shown to mediate proliferation, survival, and migration of tumor cells3.

    NRP2 knockout mice studies show that mice are viable without defects in blood vessels but display abnormal lymphatic development and defects of the central and peripheral nervous systems2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NRP2 from mouse, rat and human samples.

    For research purposes only, not for human use
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