- Peptide (C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat SERT (Accession P31652). 4th extracellular loop.
- Rat and mouse brain membranes and rat pheochromocytoma PC12 cell lysates (1:200).
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1-2. Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004), (1:200).
3-4. Anti-Serotonin Transporter (SERT) (extracellular) Antibody, preincubated with Serotonin Transporter/SERT (extracellular) Blocking Peptide (#BLP-MT004).
- Rat and mouse brain sections (frozen), (1:400).
- 5 µg antibody/1x106 human Jurkat acute T-cell leukemia cells.
- The blocking peptide is not suitable for this application.
- Live intact rat pheochromocytoma PC12 cells (1:100).
Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3. The intensity of monoaminergic signaling is determined by the availability of the monoamine, which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin (5-HT) and noradrenaline respectively, and recycling them back for release3-5.
DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. Like its counterparts, SERT’s intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization6.
SERTs are evidently expressed in serotonergic neurons, and are also expressed in various peripheral tissues including specialized cells of the gut, placenta, lung, blood lymphocytes and platelets6.
SERT knockout mice are viable and as a consequence exhibit increased extracellular levels of 5-HT and significantly decreased levels of brain tissue 5-HT due to deficient recycling and re-accumulation of serotonin by SERT7. Phenotypes displayed by SERT knockout mice include stress sensitivity, obesity and various behavioral changes. 8