This product has recently been released.
Cat #: ANT-009-F
This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
Reactivity: h, m, r
Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin (Accession Q99523). Extracellular domain.
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, Rat – identical.
Purity Affinity purified on immobilized antigen.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution 100 µl double distilled water (DDW).
Control antigen storage after reconstitution -20°C.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-009), and direct flow cytometry (labeled antibody).
May also work in: ic, lci
Direct flow cytometry
Mouse J774 macrophage and human THP-1 monocytic leukemia cells (2.5 µg).
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Lyophilized PowderControl Antigen Included
Last update: 21/01/2019
For research purposes only, not for human use