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Anti-Sortilin (extracellular) Antibody

SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Cat #: ANT-009
Alternative Name SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin (Accession Q99523). Extracellular domain.
    Accession (Uniprot) Number Q99523
    Gene ID 6272
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - identical.
    RRID AB_2040216.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.4 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, lci, wb
    May also work in: ic*, ip*
    Western blot
    • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
      1,2. Anti-Sortilin (extracellular) Antibody (#ANT-009), (1:400).
      3,4. Anti-Sortilin (extracellular) Antibody, preincubated with Sortilin (extracellular) Blocking Peptide (#BLP-NT009).
    • Expression of Sortilin in rat neocortex
      Expression of Sortilin in rat neocortex
      Immunohistochemical staining of rat neocortex with Anti-Sortilin (extracellular) Antibody (#ANT-009). A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with mouse anti-parvalbumin (PV, green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.
    Indirect flow cytometry
    • Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells:
      Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Sortilin (extracellular) Antibody (#ANT-009), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Petersen, C.M. et al. (1997) J. Biol. Chem. 272, 3599.
    2. Mazella, J. et al. (1998) J. Biol. Chem. 273, 26273.
    3. Nykjaer, A. et al. (2004) Nature 427, 843.
    4. Teng, H.K. et al. (2005) J. Neurosci. 25, 5455.
    Scientific background

    Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has lately been identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.

    Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in the brain, spinal cord, skeletal muscle and adipocytes.

    Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90% of sortilin is retained in intracellular compartments with only about 10% reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Sortilin (extracellular) Antibody
    Knockout validation of Anti-Sortilin (extracellular) Antibody in siRNA-treated human MDA-MB-231 cells.Western blot analysis of human MDA-MB-231 breast cancer cells with Anti-Sortilin (extracellular) Antibody (#ANT-009). Sortilin immunodetection is shown under non-transfected conditions, siCONT, and si SORT at different time intervals. Clear and significant down regulation of Sortilin expression is observed at 24h, while no detection is observed 72h after si SORT treatment. Adapted from Roselli, S. et al. (2015) Oncotarget 6, 10473. with permission of Impact Journals. 

    Last update: 22/01/2023

    Anti-Sortilin (extracellular) Antibody (#ANT-009) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples.

    For research purposes only, not for human use



    KO validation citations
    1. Western blot analysis of human MDA-MB-231 breast cancer cells. Also tested in siRNA treated cells.
      Roselli, S. et al. (2015) Oncotarget 6, 10473.
    Western blot citations
    1. Human MDA-MB-231 breast cancer cells. Also tested in siRNA treated cells.
      Roselli, S. et al. (2015) Oncotarget 6, 10473.
    More product citations
    1. Urban, D. et al. (2013) J. Biochem. 153, 197.


    Scientific Background

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