Anti-TRPC4 (extracellular) Antibody

Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1
    Cat #: ACC-119
    Alternative Name Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4 (Accession O35119). 2nd extracellular loop.
    • Anti-TRPC4 (extracellular) Antibody
    Accession (Uniprot) Number O35119
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2340913.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-TRPC4 (extracellular) Antibody
      Western blot analysis of rat and mouse brain lysates:
      1. Anti-TRPC4 (extracellular) Antibody (#ACC-119), (1:200).
      2. Anti-TRPC4 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-TRPC4 (extracellular) Antibody
      Expression of TRPC4 in rat brain
      Immunohistochemical staining of rat third ventricle region using Anti-TRPC4 (extracellular) Antibody (#ACC-119). A. TRPC4 staining (red) is detected along the wall of the third ventricle (horizontal arrow) and astrocytic fibers extending from the wall (vertical arrow). B. Nucleus staining using DAPI as the counterstain (blue). C. Merge images of A and B.
    Live cell imaging / Immunocytochemistry
    • Anti-TRPC4 (extracellular) Antibody
      Expression of TRPC4 in rat PC12 cells
      Cell surface detection of TRPC4 in intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPC4 (extracellular) Antibody (#ACC-119), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
    References
    1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362.
    2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    3. Clapham, D.E. (2003) Nature 426, 517.
    4. Padinjat, R. and Andrews, S. (2004) J. Cell Sci. 117, 5707.
    5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
    6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337.
    Scientific background

    The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.

    The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:
    1) TRPC1
    2) TRPC4 and TRPC5
    3) TRPC3, TRPC6, TRPC7
    4) TRPC2.2,5
    They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

    TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by Ca2+ store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-TRPC4 (extracellular) Antibody (#ACC-119) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPC4 from mouse, rat, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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