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Anti-TRPC1 (extracellular) Antibody

Short transient receptor potential channel 1, Transient receptor protein 1, TRP1

Cat #: ACC-118
Alternative Name Short transient receptor potential channel 1, Transient receptor protein 1, TRP1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1 (Accession Q9QX01). 2nd extracellular loop.
Accession (Uniprot) Number Q9QX01
Gene ID 89821
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2340912.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-TRPC1 (extracellular) Antibody (#ACC-118), (1:200).
    2. Anti-TRPC1 (extracellular) Antibody, preincubated with TRPC1 (extracellular) Blocking Peptide (#BLP-CC118).
Indirect flow cytometry
  • Cell surface detection of TRPC1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    Cell surface detection of TRPC1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-TRPC1 (extracellular) Antibody (#ACC-118), (2.5μg) + goat-anti-rabbit-FITC.
Live cell imaging / Immunocytochemistry
  • Expression of TRPC1 in PC12 cells
    Expression of TRPC1 in PC12 cells
    Cell surface detection of TRPC1 in intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC1 (extracellular) Antibody (#ACC-118), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining with DAPI as the counterstain. C. Merged images of A and B.
References
  1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362.
  2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
  3. Clapham, D.E. (2003) Nature 426, 517.
  4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707.
  5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
  6. Liu, X. et al. (2003) J. Biol. Chem. 278, 11337.
Scientific background

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.

The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities:
1.) TRPC1 2.) TRPC4 and TRPC5 3.) TRPC3TRPC6TRPC7 4.) TRPC22,5. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

TRPC1 was the first mammalian TRP protein that was reported to form an ion channel2. It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established as yet1-3.

The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+, store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2022

Anti-TRPC1 (extracellular) Antibody (#ACC-118) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize TRPC1 from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Live cell imaging citations
  1. Live intact mouse oligodendrocyte progenitor cells (1:100).
    Paez, P.M. et al. (2011) J. Neurosci. 31, 3625.
More product citations
  1. Ng, L.C. et al. (2012) Am. J. Physiol. 303, C1156.

Specifications

Scientific Background

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