Anti-TRPC1 (extracellular) Antibody

Short transient receptor potential channel 1, Transient receptor protein 1, TRP1
    Cat #: ACC-118
    Alternative Name Short transient receptor potential channel 1, Transient receptor protein 1, TRP1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1 (Accession Q9QX01). 2nd extracellular loop.
        Anti-TRPC1 (extracellular) Antibody
    Accession (Uniprot) Number Q9QX01
    Gene ID 89821
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - identical.
    RRID AB_2340912.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, lci, wb
    May also work in: ifc*, ih*, ip*
      • Anti-TRPC1 (extracellular) Antibody
        Western blot analysis of rat brain lysate:
        1. Anti-TRPC1 (extracellular) Antibody (#ACC-118), (1:200).
        2. Anti-TRPC1 (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-TRPC1 (extracellular) Antibody
        Expression of TRPC1 in PC12 cells
        Cell surface detection of TRPC1 in intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC1 (extracellular) Antibody (#ACC-118), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining with DAPI as the counterstain. C. Merged images of A and B.
    References
      • The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.

        The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities:
        1.) TRPC1 2.) TRPC4 and TRPC5 3.) TRPC3TRPC6TRPC7 4.) TRPC22,5. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

        TRPC1 was the first mammalian TRP protein that was reported to form an ion channel2. It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established as yet1-3.

        The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+, store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Anti-TRPC1 (extracellular) Antibody (#ACC-118) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPC1 from mouse, rat, and human samples.

    For research purposes only, not for human use
    Citations
      • Live intact mouse oligodendrocyte progenitor cells (1:100).
        Paez, P.M. et al. (2011) J. Neurosci. 31, 3625.
      • Ng, L.C. et al. (2012) Am. J. Physiol. 303, C1156.
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