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Anti-TRPC5 Antibody

Short transient receptor potential channel 5, Transient receptor protein 5, TRP5

Cat #: ACC-020
Alternative Name Short transient receptor potential channel 5, Transient receptor protein 5, TRP5
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5 (Accession Q9UL62). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9UL62
    Gene ID 7224
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, rabbit - identical.
    RRID AB_2040241.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Western blot analysis of rat brain membranes: 
      Western blot analysis of rat brain membranes: 
      1. Anti-TRPC5 Antibody (#ACC-020), (1:200).   
      2. Anti-TRPC5 Antibody, preincubated with TRPC5 Blocking Peptide (#BLP-CC020).
    Immunoprecipitation
    • Rat neonatal ventricular cardiomyocytes (Sabourin, J. et al. (2016) J. Biol. Chem. 291, 13394.).
    Immunohistochemistry
    • Expression of TRPC5 in rat cortex
      Expression of TRPC5 in rat cortex
      Immunohistochemical staining of rat cortex using Anti-TRPC5 Antibody (#ACC-020). A. TRPC5 immunoreactivity (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
    • Rat substantia nigra (De March, Z. et al. (2006) Neurosci. Lett. 402, 35.).
    Immunocytochemistry
    • Human U373 MG cells (1:120) (Barajas, M. et al. (2008) J. Neurosci. Res. 86, 3456.).
    References
    1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362.
    2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    3. Clapham, D.E. (2003) Nature 426, 517.
    4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707.
    5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
    6. Liu, X. et al. (2003) J. Biol. Chem. 278, 11337.
    Scientific background

    The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4

    The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:
    1) TRPC1
    2) TRPC4 and TRPC5
    3) TRPC3, TRPC6, TRPC7
    4) TRPC2.2,5
    They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

    TRPC5 channels are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells, and can be activated either by calcium store depletion or by GPCR stimulation pathways.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-TRPC5 Antibody
    Expression of TRPC5 in rat nodose ganglion.Immunohistochemical staining of rat nodose ganglion sections using Anti-TRPC5 Antibody (#ACC-020). TRPC5 staining (green) is detected in the somata of sensory neurons. TRPC5 staining co-localizes with PGP9.5 neuronal marker. Shown is a control experiment without the primary antibody.Adapted from Lau, O.C. et al. (2016) Nat. Commun. 7, 11947. with permission of Nature Publishing Group.
    Last update: 24/01/2021

    Anti-TRPC5 Antibody (#ACC-020) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPC5 from mouse, rat, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of mouse primary mesenteric endothelial cell (MMEC) lysate. Tested in TRPC5-siRNA treated cells.
      Zhu, Y. et al. (2019) J. Biol. Chem. 294, 28.
    Western blot citations
    1. Mouse primary mesenteric endothelial cell (MMEC) lysate. Also tested in TRPC5-siRNA treated cells.
      Zhu, Y. et al. (2019) J. Biol. Chem. 294, 28.
    2. Rat pulmonary artery lysate.
      Jiang, H.N. et al. (2016) Biomed. Pharmacol. 82, 20.
    3. Rat neonatal ventricular cardiomyocytes (1:200).
      Sabourin, J. et al. (2016) J. Biol. Chem. 291, 13394.
    Immunoprecipitation citations
    1. Rat neonatal ventricular cardiomyocytes.
      Sabourin, J. et al. (2016) J. Biol. Chem. 291, 13394.
    Immunohistochemistry citations
    1. Rat substantia nigra.
      De March, Z. et al. (2006) Neurosci. Lett. 402, 35.
    Immunocytochemistry citations
    1. Rat primary cultured fetal and neonatal ventricular myocytes (1:100).
      Jiang, Y. et al. (2014) Cell Tissue Res. 355, 201.
    2. Human U373 MG cells (1:120).
      Barajas, M. et al. (2008) J. Neurosci. Res. 86, 3456.
    More product citations
    1. Lau, O.C. et al. (2016) Nat. Commun. 7, 11947.
    2. He, Z. et al. (2012) J. Neurosci. 32, 9383.
    3. Yoshida, J. et al. (2005) Eur. J. Pharmacol. 510, 217.
    4. Facemire, C.S. et al . (2004) Am. J. Physiol. 286, F546.
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