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Guinea pig Anti-TRPV1 (VR1) Antibody

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
Cat #: AGP-118
Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Accession (Uniprot) Number O35433
Gene ID 83810
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.
RRID AB_2721813.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of Rat DRG lysate:
    Western blot analysis of Rat DRG lysate:
    1. Guinea pig Anti-TRPV1 (VR1) Antibody (#AGP-118), (1:200).
    2. Guinea pig Anti-TRPV1 (VR1) Antibody preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).

    Following a broad screen of secondary antibodies, the following were used for these applications: Western blot analysis: #106-035-006 (Jackson ImmunoResearch).
Immunohistochemistry
  • Expression of TRPV1 in rat DRGs
    Expression of TRPV1 in rat DRGs
    Immunohistochemical staining of TRPV1 in rat dorsal root ganglion (DRG) using Guinea pig Anti-TRPV1 (VR1) Antibody (#AGP-118), (1:200). A. TRPV1 staining (red) appears in some DRG cells (arrows). B. DAPI counterstain (blue) is used to stain nuclei. C. Merged image of panels A and B.
References
  1. Liao, M. et al. (2013) Nature 504, 107.
  2. Sanz-Salvador, L. et al. (2011) J. Biol. Chem. 287, 19462.
  3. Hanack, C. et al. (2015) Cell 160, 759.
Scientific background

TRP channels represent a large and diverse family of non-selective cation channels that respond to a wide range of chemical and physical stimuli. TRPV1 (transient receptor potential vanilloid) is a non-selective cation channel, highly permeable to Ca2+.

TRPV1 is comprised of four subunits arranged around a central ion permeation pore. Each subunit consists of six transmembrane α-helices (S1–S6) that span the lipid bilayer and a re-entrant loop with a pore helix located between S5 and S6. The outer pore region of TRPV1 is wide open compared to KV channels, with a broad funnel-like structure that probably enhances accessibility to both small and large pharmacophores.

TRPV1 is the receptor for capsaicin, the pungent agent from chili peppers. TRPV1 is activated by noxious heat, elicits burning pain and is modulated by inflammatory agents, such as extracellular protons and bioactive lipids, which contribute to pain hypersensitivity1. TRPV1 activation by capsaicin is followed by nociceptor desensitization, is markedly depending on Ca2+ and involves various intracellular signaling pathways. Subsequently, dephosphorylation by the phosphatase calcineurin of TRPV1 previously phosphorylated by protein kinase A (PKA) or Ca2+-calmodulin-dependent kinase II leads to TRPV1 desensitization2.

Recently, the GABA(B) R1 receptor subunit has been identified as an inhibitor of TRPV1 sensitization in the context of diverse inflammatory settings. The endogenous GABA(B) agonist, GABA, is released from nociceptive nerve terminals, suggesting an autocrine feedback mechanism limiting TRPV1 sensitization. The effect of GABA(B) on TRPV1 is independent of canonical G protein signaling and rather relies on close juxtaposition of the GABA(B) R1 receptor subunit and TRPV13.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TRPV1 channel. Guinea pig Anti-TRPV1 (VR1) Antibody (#AGP-118), raised in guinea pigs, can be used in western blot analysis. It has been designed to recognize TRPV1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV1 (VR1) Antibody (#ACC-030) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse brain lysate (1:1000).
    Li, M. et al. (2020) Biochem. Biophys. Res. Commun. 521, 868.
Immunohistochemistry citations
  1. Mouse brain sections (1:500).
    Li, M. et al. (2020) Biochem. Biophys. Res. Commun. 521, 868.
Immunofluorescence citations
  1. Mouse brain sections (1:500).
    Li, M. et al. (2020) Biochem. Biophys. Res. Commun. 521, 868.
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