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Anti-ZnT4 (SLC30A4) Antibody

Zinc transporter 4, Solute carrier family 30 member 4

Cat #: AZT-014
Alternative Name Zinc transporter 4, Solute carrier family 30 member 4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DEVSDEGLSRFNKLR, corresponding to amino acid residues 33 - 47 of rat SLC30A4 (Accession O55174). Intracellular, N-terminus
Accession (Uniprot) Number O55174
Gene ID 64469
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; Human - 13 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
ZnT4 (SLC30A4) Blocking Peptide (#BLP-ZT014)
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:500).
    3-4. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
  • Western blot analysis of rat small intestine lysate:
    Western blot analysis of rat small intestine lysate:
    1. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:400).
    2. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
  • Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):
    Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):
    1-2. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:500).
    3-4. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
Immunohistochemistry
  • Expression of ZnT4 in mouse hypothalamus. 
    Expression of ZnT4 in mouse hypothalamus. 
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. ZnT4 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014), suppressed staining. Cell nuclei are stained with DAPI (blue).  
References
  1. Xu, Y. et al. (2019) Mol Brain12, 106.
  2. Hara T. et al. (2017) J Physiol Sci. 67, 283.
  3. Portbury, S. D. et al. (2017) Int J Mol Sci. 18, 2506.
  4. Willekens J. et al. (2022) Nutrients. 14, 2526.
Scientific background

ZnT4 (also known as Zinc transporter 4 and SLC30A4) is a member of the ZnT family of zinc transporters.

Zinc transporters tightly regulate zinc homeostasis. Zinc participates in physiological functions such as DNA synthesis, neurotransmission and brain development. Protein-bound zinc stabilizes and functionalizes proteins, while free zinc acts as a signaling molecule. Zinc defficiency leads to mental lethargy, growth retardation and immune dysfunction, while zinc overload can affect the function of lymphocytes and cause copper deficiency. 1

Zinc transporters are classified into two families: The ZnT (SLC30) family and the ZIP (SLC39) family. Most ZnT proteins form homodimers and have six transmembrane domains, with a long His-rich loop between these domains, which may function as a sensor of cytosolic zinc levels. The C- and N- termini of ZnTs are located inside the cytoplasm. ZnT proteins are efflux transporters that reduce cytosolic zinc by transporting it out of the cell or into intracellular compartments. In contrast, ZIP proteins are influx transporters that form homodimers or hetrodimers. They have eight transmembrane domains, and their C- and N- termini are both located outside the plasma membrane or in the lumen of a subcellular compartment.2

ZnT4 is widely expressed in humans, with notable enrichment in the brain as well as several other organs, including the thyroid, lung, testis, heart, skin and pancreas. ZnT4 is expressed in endosomes/lysosomes, the Golgi apparatus and cytoplasmic vesicles, where it is involved in vesicular secretory functions.3

There is increasing evidence that zinc transporters are connected with the formation of senile plaques in Alzheimer's Disease (AD). Abundant expression and altered distribution of ZnT4 was present in the entire body of the senile plaques of APP/PS1 mice and human AD brains.1 Also, it has been shown that ZnT4 mutant mice develop balance and spatial memory behavioral abnormalities.4

Expression of ZnT4 in the mammary gland has been reported in mouse, where it has been shown to cause the lethal milk syndrome (LMS). Mice with LMS are not zinc deficient but females cannot produce milk containing sufficient zinc for the pups to survive. 4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 10/01/2023

Anti-ZnT4 (SLC30A4) Antibody (#AZT-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT4 from rat, mouse and human samples.

For research purposes only, not for human use
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