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Certificate of Analysis

ASIC Channel Antibody Explorer Kit

A Screening Package of ASIC Channel Antibodies Economically Priced

Cat #: AK-226
Size: 16 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-ASIC1 Antibody Cat #: ASC-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
Homology
Human - identical; mouse - 19/20 amino acid residues identical.

ASIC1 Blocking Peptide Cat #: BLP-SC014

Size: 40 µg

Guinea pig Anti-ASIC1 Antibody Cat #: ASC-014-GP (formerly AGP-053)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
Homology
Human - identical; mouse - 19/20 amino acid residues identical.

ASIC1 Blocking Peptide Cat #: BLP-SC014

Size: 40 µg

Anti-ASIC2a Antibody Cat #: ASC-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a (Accession Q16515). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

ASIC2a Blocking Peptide Cat #: BLP-SC012

Size: 40 µg

Anti-ASIC3 Antibody Cat #: ASC-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

ASIC3 Blocking Peptide Cat #: BLP-SC018

Size: 40 µg

Guinea pig Anti-ASIC3 Antibody Cat #: ASC-018-GP (formerly AGP-052)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

ASIC3 Blocking Peptide Cat #: BLP-SC018

Size: 40 µg

Anti-ASIC4 Antibody Cat #: ASC-015

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide CKIKFAEEDAKPKEKEAGDE, corresponding to amino acid residues 7-26 of rat ASIC4 (Accession Q9QYV9).  Intracellular, N-terminus.
Homology
Mouse, human - identical.

ASIC4 Blocking Peptide Cat #: BLP-SC015

Size: 40 µg

Anti-ASIC5 (extracellular) Antibody Cat #: ASC-032

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5 (Accession Q9R0Y1). Extracellular loop.
Homology
Rat, human – 13/14 amino acid residues identical.

ASIC5 (extracellular) Blocking Peptide Cat #: BLP-SC032

Size: 40 µg

Anti-pan ASIC (extracellular) Antibody Cat #: ASC-031

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1 (Accession P55926), amino acid residues 367–381 of rat ASIC2 (Accession Q62962), amino acid residues 375–388 of rat ASIC3 (Accession O35240) and amino acid residues 378–390 of rat ASIC4 (Accession Q9JHS6). Extracellular loop.
Homology
ASIC1: Mouse, rat, human – identical.
ASIC2: Mouse, rat, human – 14/15 amino acid residues identical.
ASIC3: Mouse, rat, human – 12/15 amino acid residues identical.
ASIC4: Mouse, rat – 12/15 amino acid residues identical; human – 11/15 amino acid residues identical.

Pan ASIC (extracellular) Blocking Peptide Cat #: BLP-SC031

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#ASC-014-GP & #ASC-018-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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