Cono RPRF-amide

A Specific Potentiator of ASIC3 Channel
    Cat #: STC-170
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 574 Da.
    Purity: >98% (HPLC)
    Form Lyophilized powder.
    Effective concentration 50 µM.
    Sequence RPRF.
    Modifications C-terminal amidation at Phe4.
      • Cono RPRF-amide
    Molecular formula C26H43N11O4.
    Activity Cono RPRF-amide is an ASIC3 channel positive modulator1.
      • Reimers, C. et al. (2017) Proc. Natl. Acad. Sci. U. S. A. 114, E3507.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Cono RPRF-amide
        Alomone Labs Cono RPRF-amide positively modulates ASIC3 channels expressed in Xenopus oocytes.
        A. Representative time course of Cono RPRF-amide (#STC-170) potentiation of ASIC3 current. Membrane potential was held at -60 mV, current was elicited every 100 sec by a transient application of pH 5.5, and reversibly potentiated by a 60 sec application of 50 µM Cono RPRF-amide, as indicated. B. Superimposed traces of ASIC3 currents before (black) and following (green) application of 50 µM Cono RPRF-amide, which significantly extends the current following stimulation (arrow). Taken from the experiment in A.
        Cono RPRF-amide
        Alomone Labs Cono RF-amide peptides positively modulate ASIC3 channels expressed in Xenopus oocytes.
        Representative superimposed traces of ASIC3 currents before (black) and following a 60 sec application of 50 µM Cono RPRF-amide (#STC-170), Cono VGRPRF-amide (#STC-172) or Cono AIVGRPRF-amide (#STC-174) (green), which significantly extend the current evoked by a transient application of pH 5.5 (arrow), at membrane potential of -80 mV.
    References - Scientific background
    • 1. Reimers, C. et al. (2017) Proc. Natl. Acad. Sci. U. S. A. 114, E3507.
    • 2. Osmakov, D.I. et al. (2017) Front. Mol. Neurosci. 10, 282.
      • Cono RPRF-amide conotoxin is a peptide toxin originally isolated from the venom of the cone snail Conus textile. It strongly potentiates ASIC3 by directly binding to the channel, thus modulating the proton-activated current. Cono RPRF-amide belongs to the subgroup of cono-RFamides containing C-terminal sequence of four amino acids and a C-terminal Arg–Phe–NH2 (RFa) motif. Research shows that injection of the cono-RFamide in mice muscle enhanced acid-induced muscle pain1.

        ASIC channels are proton-gated Na+ channels directly activated by extracellular protons. They are expressed throughout the CNS and the peripheral nervous system. They are responsible for synaptic transmission and detection of painful acidosis. ASICs play an important role in several physiological and pathological conditions including synaptic plasticity and learning, fear conditioning, and neurodegeneration associated with ischemic stroke1,2.

    Target ASIC3 channel
    Net Peptide Content: 100%
    Last update: 20/08/2019

    Cono RPRF-amide (#STC-170) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use