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Guinea Pig Anti-GAP43 Antibody

Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Cat #: ANR-016-GP
Alternative Name Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43 (Accession P07936). C-terminus.
Accession (Uniprot) Number P07936
Gene ID 29423
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 13 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
GAP43 Blocking Peptide (#BLP-NR016)
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:400).
    3, 4. Guinea Pig Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:400).
    2. Guinea Pig Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
Immunohistochemistry
  • Expression of GAP43 in rat hippocampus.
    Expression of GAP43 in rat hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:300), followed by goat anti-guinea pig - Alexa Fluor-594. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (red) in the subgranular layer (arrows), over the granule layer (G) and over the outer molecular layer (OML).  B. Pre-incubation of the antibody with GAP43 Blocking Peptide (#BLP-NR016), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Li, Y. et al. (2017) Braz. J. Med. Biol. Res. 50, e5561.
  2. Grasselli, G. and Strata, P. (2013) Front. Neural Circuits. 7, 25.
Scientific background

Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.

GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from mouse, rat and human samples.

The antigen used to immunize guinea pigs is the same as Anti-GAP43 Antibody (#ANR-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
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