- Peptide (C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 of rat TrkB (Accession Q63604). Extracellular, N-terminus.
TrkB (extracellular) Blocking Peptide (#BLP-NT019)
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse Neuro-2a neuroblastoma cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):1-3. Guinea Pig Anti-TrkB (extracellular) Antibody (#ANT-019-GP), (1:200).
4-6. Guinea Pig Anti-TrkB (extracellular) Antibody, preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
- Expression of TrkB in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-TrkB (extracellular) Antibody (#ANT-019-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. TrkB immunoreactivity (red) appears in purkinje cells (arrows) and diffusely in the molecular layer (MOL). B. Pre-incubation of the antibody with TrkB (extracellular) Blocking Peptide (#BLP-NT019), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer, G = granule layer.
- Expression of TrkB in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-TrkB (extracellular) Antibody (#ANT-019-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. TrkB immunoreactivity (red) appears in the superficial layer of the spinal cord dorsal horn (arrows). B. Pre-incubation of the antibody with TrkB (extracellular) Blocking Peptide (#BLP-NT019), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Webster, N.J.G. and Pirrung, M.C. (2008) BMC Neurosci. 9, S1.
- Quartu, M. et al. (2003) Int. J. Dev. Neurosci. 21, 309.
- Aoki, C. et al. (2000) J. Neurosci. Res. 59, 454.
- Yan, Q. et al. (1997) J. Comp. Neurol. 378, 135.
- Patapoutian, A. and Reichardt, L.F. (2001) Curr. Opin. Neurobiol. 11, 272.
- Schecterson, L.C. and Bothwell, M. (2010) Develop. Neurobiol. 70, 332.
- Grimes, M.L. et al. (1996) J. Neurosci. 16, 7950.
BDNF and NT-4 belong to the neurotrophin family which also includes NGF and NT-3. These neurotrophins bind two groups of receptors. The p75NTR receptor is common to all four neurotrophins and is a member of the tumor necrosis factor receptor family. The tropomyosin-related kinase (Trk) receptors are receptor tyrosine kinases (RTKs) and three receptors form this family: TrkA, TrkB, and TrkC1.
As mentioned above, the p75NTR receptor binds to all neurotrophins with similar affinities while the Trk receptors are the ones to display the selectivity for the neurotrophins. TrkA is activated by NGF binding, TrkB by that of BDNF and NT-4, while TrkC is stimulated by the binding of NT-31.
All three Trk receptors are highly expressed in the mammalian brain in very distinct regions and are also expressed in the peripheral nervous system2-4. Cholinergic neurons in the basal forebrain exclusively express TrkA, while TrkB and TrkC are highly expressed in the hippocampus. Motor and sensory neurons in the peripheral nervous system express Trk receptors. Interestingly, Trk receptors are not essential for development, but knockout mice die shortly after birth. Indeed, TrkB-deficient mice demonstrate a significant decrease in motor neurons and synaptogenesis1.
Trk receptors have many motifs in the extracellular region, including cell-adhesion domains, three tandem leucine rich motifs flanked by two clusters of cysteines. In the membrane proximal region of the receptor there are also two immunoglobulin-like domains5. The binding of neurotrophins to Trk receptors promotes receptor dimerization resulting in kinase activation. Activated Trk receptors then phosphorylate a cascade of signaling molecules including the Ras/ERK, PI3K/Akt pathways and PLC-g1. Activated Trk receptors also create internal docking sites for other signaling adaptor proteins to bind to5. Splice variants of TrkA, TrkB and TrkC have been observed. These splice isoforms are mainly affected in the tyrosine kinase domain of the receptor lying in the cytoplasm. Endocytosis is an important signaling trait of Trk receptors.
Following neurotrophin binding to the Trk receptor, the receptor complex is then internalized via endocytosis in order to terminate signaling. However, in the axonal compartment of neurons the internalization process of the neurotrophin complexed to the receptor is part of the signaling process and is important for activating transcription processes in the nucleus6,7.
Species reactivity key:
Guinea Pig Anti-TrkB (extracellular) Antibody (#ANT-019-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TrkB from mouse, rat and human samples.
The antigen used to immunize guinea pigs is the same as Anti-TrkB (extracellular) Antibody (#ANT-019) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.