Guinea pig Anti-TRPV4 Antibody

OTRPC4, VRL-2, VR-OAC, TRP12

Cat #: AGP-147
Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.
Accession (Uniprot) Number Q9ERZ8
Gene ID 66026
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.
RRID AB_2876796
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Guinea pig Anti-TRPV4 Antibody (#AGP-147), (1:1500).
    2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
  • Western blot analysis of rat DRG lysates:
    Western blot analysis of rat DRG lysates:
    3. Guinea pig Anti-TRPV4 Antibody (#AGP-147), (1:200).
    4. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
References
  1. Montell, Cet al. (2002) Mol. Cell9, 229.
  2. Clapham, D.E. (2003) Nature 426, 517.
  3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.
  4. Clapham, D.Eet al. (2003) Pharmacol. Rev55, 591.
  5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
  6. Peng, J.Bet al. (2003) J. Physiol551.3, 729.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR, the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPCTRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPATRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/11/2021

Guinea pig Anti-TRPV4 Antibody (#AGP-147), raised in guinea pig, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPV4 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV4 Antibody (#ACC-034) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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