Kv4.2 Blocking Peptide (#BLP-PC023) is the original antigen used for immunization during Anti-KV4.2 Antibody (#APC-023) generation. The blocking peptide binds and ‘blocks’ Anti-Kv4.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Rat brain sections.
Human myometrium sections (1:50) (Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.).
- Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human U-87 MG glioblastoma cell lysate (lanes 3 and 6):
- Multiplex staining of DPP6 and KV4.2 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400) and Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:1000). A. DPP6 (green) is expressed in the CA3 rat hippocampal region. B. KV4.2 (red) is expressed in the same region. C. Merge of the two images demonstrates partial colocalization in neurons of the CA3 region (arrows). Cell nuclei are stained with DAPI (blue).
- Expression of KV4.2 in mouse CA1 hippocampal regionImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:400), followed by biotinylated donkey anti-guinea pig antibody and Streptavidin-Cy3. KV4.2 staining (red) appears in stratum oriens (SOR) and stratum radiatum (SR). Nuclei are labeled with DAPI (blue).
- (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881).