Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv4.2 Blocking Peptide (#BLP-PC023) is the original antigen used for immunization during Anti-KV4.2 Antibody (#APC-023) generation. The blocking peptide binds and ‘blocks’ Anti-Kv4.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV4.2 Antibody (#APC-023), (1:200).
2. Anti-KV4.2 Antibody, preincubated with Kv4.2 Blocking Peptide (#BLP-PC023).- Rat brain sections.
Human myometrium sections (1:50) (Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.). 
Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human U-87 MG glioblastoma cell lysate (lanes 3 and 6):1-3. Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:500).
4-6. Guinea pig Anti-KV4.2 Antibody, preincubated with Kv4.2 Blocking Peptide (#BLP-PC023).
Multiplex staining of DPP6 and KV4.2 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400) and Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:1000). A. DPP6 (green) is expressed in the CA3 rat hippocampal region. B. KV4.2 (red) is expressed in the same region. C. Merge of the two images demonstrates partial colocalization in neurons of the CA3 region (arrows). Cell nuclei are stained with DAPI (blue).
Expression of KV4.2 in mouse CA1 hippocampal regionImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:400), followed by biotinylated donkey anti-guinea pig antibody and Streptavidin-Cy3. KV4.2 staining (red) appears in stratum oriens (SOR) and stratum radiatum (SR). Nuclei are labeled with DAPI (blue).
Properties
Sequence
- (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881).
Accession (Uniprot) Number Q63881
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
