Anti-DPP6 (extracellular) Antibody

Dipeptidyl-Peptidase 6, Dipeptidyl aminopeptidase-like protein 6, DPPX
    Cat #: APC-146
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KKHEDESEAWLHR, corresponding to amino acid residues 414-426 of human DPP6 (Accession P42658). Extracellular, C-terminus.
    Accession (Uniprot) Number P42658
    Gene ID 1804
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
    1. Anti-DPP6 (extracellular) Antibody (#APC-146), (1:500).
    2. Anti-DPP6 (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1-2. Anti-DPP6 (extracellular) Antibody (#APC-146), (1:200).
    3-4. Anti-DPP6 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Immuno-colocalization of DPP6 and KV4.2 in rat hippocampus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400) and Guinea pig Anti-KV4.2 Antibody (#AGP-038), (1:1000). A. DPP6 (green) is expressed in the CA3 rat hippocampal region. B. KV4.2 (red) is expressed in the same region. C. Merge of the two images demonstrates partial colocalization in neurons of the CA3 region (arrows). Cell nuclei are stained with DAPI (blue).
    Expression of DPP6 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400), followed by donkey anti-rabbit-Cy3 antibody (red). Staining in the hippocampal CA3 region appears in interneurons (arrows). DAPI is used to stain nuclei (blue).
    References
    1. Lin, L. et al. (2014) J. Biol. Chem. pii: jbc.M114.578070.
    2. Jerng, H.H. and Pfaffinger, P.J. (2012) PLoS One 7, e38205.
    3. Busek, P. et al. (2004) Int. J. Biochem. Cell. Biol. 36, 408.
    4. Lin, L. et al. (2013) Nat. Commun. 4, 2270.
    5. Liao, C. et al. (2013) Eur. J. Med. Genet. 56, 484.
    6. Prontera, P. et al. (2014) Neurogenetics 15, 237.
    7. Chettier, R. et al. (2014) PLoS One 9, e103968.
    Scientific background

    Dipeptidyl-peptidase 6 (DPP6) is a type II membrane glycoprotein1 which is a part of the dipeptydil aminopeptidase-like proteins (DPPLs) subfamily. It is an auxiliary, glycoproteinic subunit of voltage dependent K+ channel 4 (KV4.x) that has a short cytoplasmic domain, a long extracellular domain and a transmembrane domain. It regulates sub-threshold, A-type K+ currents, and is important for the enhancement and localization of KV4.2 channels2.

    DPPLs are a subgroup of the larger prolyl oligopeptidase family. A loss-of-function mutation at their enzymatic serine renders them incapable of cleaving peptides. Notable DPPLs include DPP8, DPP9, and DPPIV – the latter is known to regulate several key functions in varied systems, ranging from immune to CNS1,3.

    Alternative mRNA splicing of the DPP6 gene (part of the di-genic DPLP group that also consists DPP10) gives rise to several isoforms that differ in their N-terminal (DPP6a, DPP6K, DPP6S, DPP6L DPP6E)4.

    DPP6 proteins are widely expressed throughout both of the nervous systems – albeit much more predominantly in the CNS - and are known to play a major role in neurogenesis and neurodevelopment5.

    Deletion mutations and haplo-insufficiency of the DPP6 gene has been shown to be associated with autism spectrum disorder, Gilles de la Tourette syndrome, dominant microcephaly and mental retardation5,6. Endometriosis – a condition characterized by extra-uteral endometrial glands that affects one tenth of fertile women population – has been linked with DPP6 in genome wide associations studies (GWASs)7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human DPP6. AntiDPP6 (extracellular) Antibody (#APC-146) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize DPP6 from rat, mouse and human samples.

    For research purposes only, not for human use