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Home › Products › Ion Channels › K+ Channels › Inward Rectifier K+ Channels › Blockers

Certificate of Analysis

  • Bioassay Tested
    The specificity of this antibody has been validated in a knockout (KO) or knockdown (KD) system.

Tertiapin-Q-ATTO Fluor-488

An Inward Rectifier K+ Channel Blocker Conjugated to the Fluorescent Dye ATTO-488

Back to product page SDS

Overview

Cat #: STT-170-AG
Lyophilized Powder yes
Origin Synthetic peptide
MW: 3195.5 Da
Purity: >95% (HPLC)
Effective concentration 50-200 nM.
Modifications Disulfide bonds between Cys3-Cys14, Cys5-Cys18. Lys21 - C-terminal amidation.
Label ATTO-488. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO-488 maximum absorption is 501 nm and maximum fluorescence is at 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. The extent of labeling is one molecule of dye per molecule of Tertiapin-Q.
Structure
Activity Tertiapin-Q blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 and GIRK, but with no effect on Kir2 family members1,2. In addition, it was shown to inhibit acetylcholine induced K+ currents in the heart3,4.
References-Activity
  1. Jin, W. and Lu, Z. (1998) Biochemistry 37, 13291.
  2. Jin, W. et al. (1999) Biochemistry 38, 14294.
  3. Drici, M.D. et al. (2000) Br. J. Pharmacol. 131, 569.
  4. Kitamura, H. et al. (2000) Pharmacol. Exp. Therap. 293, 196.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Avoid exposure to light.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min). Avoid exposure to light. The product is lyophilized in 0.5 ml conical vial.
Storage of solutions Up to two weeks at 4°C or three months at -20°C. Avoid exposure to light.
Our bioassay
  • Alomone Labs Tertiapin-Q-ATTO Fluor-488 inhibits Kir3.2 channels heterologously expressed in Xenopus oocytes.
    Alomone Labs Tertiapin-Q-ATTO Fluor-488 inhibits Kir3.2 channels heterologously expressed in Xenopus oocytes.
    A continuous current trace recorded at a holding potential of -80 mV. Kir3.2 currents are downward reflections activated by high K+ containing solution. While activated, 50 nM and 100 nM of Tertiapin-Q-ATTO Fluor-488 (#STT-170-AG) were applied for 2 min (indicated as bars).
  • Alomone Labs Tertiapin-Q-ATTO Fluor-488 binds GIRK1/4-Cherry transfected HEK293T cells.
    Alomone Labs Tertiapin-Q-ATTO Fluor-488 binds GIRK1/4-Cherry transfected HEK293T cells.
    Transfected cells were incubated in the presence of 200 nM Tertiapin-Q-ATTO Fluor-488 (#STT-170-AG). The labeled toxin accumulates on the membrane surface after 26 sec. No binding is achieved in untransfected cells (data not shown).
    The pictures are a kind gift from the lab of Prof. Eithan Reuveny, Weizmann Institute, Israel.
Scientific background

Tertiapin, the native toxin, was originally isolated from European honey bee Apis mellifera venom. Native and synthetic Tertiapin blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 (Kir1.1, IC50 = 2 nM) and GIRK (Kir3 family, IC50 for the Kir3.1/3.4 heteromer was 8.6 nM) but with no effect on the Kir2 family member1. In accordance, it was shown to inhibit acetylcholine induced K+ currents in mammalian cardiomyocytes2,3.

Tertiapin-Q is a derivative of Tertiapin in which Met13 is substituted by a Gln residue. However, unlike native Tertiapin, Tertiapin-Q is non-oxidizable and therefore is more stable4.

Tertiapin-Q inhibits the above-mentioned channels with similar affinities and also inhibits Ca2-activated large conductance BK-type K+ channels in a concentration and voltage-dependent manner5.

Target Kir1.1, Kir3.2 K+ channels
Peptide Content: 100%
Lyophilized Powder
Image & Title

Tertiapin-Q-ATTO Fluor-488Double staining of GIRK2 channels in substantia nigra pars compacta using Tertiapin-Q-ATTO Fluor-488 and Guinea pig Anti-GIRK2 (Kir3.2) Antibody.Rat brain sections were first incubated with 33 nM Tertiapin-Q-ATTO Fluor-488 (#STT-170-AG), (green). The same sections were incubated with Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#APC-006-GP). A. Tertiapin-Q-ATTO Fluor-488 binding appears in profiles of substantia nigra pars compacta (SNC) neurons (arrows). B. GIRK2 staining (red) is detected in profiles of SNC neurons (arrows). C. Merge of the two images demonstrates co-staining of GIRK2 channels by Tertiapin-Q-ATTO Fluor-488 and by Guinea pig Anti-GIRK2 (Kir3.2) Antibody. DAPI staining (blue) is used to stain nuclei.

For research purposes only, not for human use
Last Update: 10/04/2023

Specifications

Citations

Citations

Applications

Scientific Background

Specific Control Product

  • Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

    Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

    Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

Related Products

Antibodies

  1. Anti-KCNJ1 (Kir1.1) Antibody (#APC-001)
  2. Anti-GIRK1 (Kir3.1) Antibody (#APC-005)
  3. Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody (#ALM-031)
  4. Anti-GIRK2 (Kir3.2) Antibody (#APC-006)
  5. Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#APC-006-GP (formerly AGP-013))

Pharmacological tools

Blockers/Antagonists: peptides/peptide toxins
  1. Tertiapin-Q (#STT-170)
  2. Tertiapin-Q-ATTO Fluor-633 (#STT-170-FR)
  3. Tertiapin-LQ (#STT-220)
  4. Tertiapin (#STT-250)
Blockers/Antagonists: small molecules
  1. VU590 dihydrochloride (#V-130)
  2. VU591 (#V-125)
Activators/Agonists: small molecules
  1. VU0529331 (#V-155)

Explorer kits & Research packs

Explorer kits
  1. Kir Channel Blocker Explorer Kit (#EK-112)
  2. K+ Channel Blockers for Pain Research Explorer Kit (#EK-390)

Shipping and Ordering Information

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