Antibodies

The control antigen

Live cell imaging with “extracellular” antibodies

Antibodies developed for immuno-colocalization:
ATTO-conjugated antibodies   |   Guinea pig-raised polyclonals

Live cell flow cytometry with FITC-conjugated antibodies

 

Alomone Labs antibodies are mostly polyclonal and are raised in rabbit and guinea pig.

We choose the epitope for immunization very carefully, taking into account two main parameters: 

✓ Immunogenicity
✓ Specificity of the epitope

Before immunization, we run a search (blast) to verify that the chosen epitope has maximum homology with other species and minimum homology between members of the same family, or other proteins.

All antibodies are affinity purified on immobilized antigens (the injected peptides). Antibodies are characterized and validated in-house by western blot analysis. All lots are tested and undergo strict quality control.

 

Reliability, reproducibility & results

 

The control antigen

The control antigen, is the peptide injected to immunize animals. We routinely use the control antigen to determine antibody specificity. We believe in the quality and reproducibility of our Antibodies. Therefore, for each polyclonal antibody, we also provide you with the control antigen (FREE of charge). We encourage you to use the control antigen in your experiments.

Learn more about how to use the control antigen

 

Live cell imaging with “extracellular” antibodies

Extracellular antibodies recognize proteins through extracellular domains. You can monitor cell movement, study protein transport across the cell, and investigate protein internalization.

✓ Work with live cells
✓ Cell permeabilization is unnecessary
✓ Cell fixation not required
✓ Cell-surface detection of protein

Learn more about our extracellular antibodies

 

Expression of α1B-Adrenoceptor in GH3 cells.Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018)

 

 

Expression of Noradrenaline Transporter (NET) in live intact rat PC12 cells.Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002)

 


 

Innovative platforms

 

Antibodies developed for immuno-colocalization

Alomone Labs has developed two innovative platforms for studying protein-protein interaction and/or protein co-localization:

ATTO-conjugated antibodies

Primary antibodies conjugated to ATTO fluorescent dyes: These antibodies are developed for immunohistochemistry (IHC) and immunocytochemistry (ICC) applications.

✓ Direct immuno-fluorescence
✓ Secondary antibodies are not necessary
✓ Ideal for immuno-colocalization with same species/host antibodies

Learn more about our ATTO-conjugated antibodies

 


Immuno-colocalization of mGluR1 and TRPC3 in mouse cerebellum.Anti-mGluR1 (extracellular)-ATTO-488 Antibody (#AGC-006-AG)


Immuno-colocalization of CaV1.2 and GABA(A) α1 Receptor in rat cerebellum.Anti-GABA(A) α1 Receptor (extracellular)-ATTO-488 Antibody (#AGA-001-AG)

 


 

Guinea pig-raised polyclonal antibodies

The majority of commercially available primary antibodies are rabbit polyclonals or mouse monoclonals. Guinea pig-raised polyclonal antibodies offer greater flexibility in multi-immunostaining. 

Learn more about our guinea pig-raised polyclonal antibodies portfolio

 


Immuno-colocalization of GluN1 and Plexin-A1 in rat olfactory bulb.Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046)


Immuno-colocalization of Kir6.2 and BDNF in mouse hippocampus.Guinea pig Anti-Kir6.2 Antibody (#AGP-067)

 


 

Live cell flow cytometry with FITC-conjugated antibodies

Antibodies conjugated to FITC dye are specifically developed for direct flow cytometry. They undergo testing with appropriate isotype controls. The FITC dye is conjugated to “extracellular” antibodies enabling live cell detection of proteins.

✓ Secondary antibodies are not necessary
✓ Time saving
✓ Works with live cells
✓ Cell permeabilization is unnecessary
✓ Cell fixation not required
✓ Cell-surface detection of protein

Learn more about our FITC-conjugated antibodies

 


Direct flow cytometry of live intact human THP-1 monocytic leukemia cells.Anti-SLC7A2 (extracellular)-FITC Antibody (#ANT-103-F)


Direct flow cytometry of live intact human THP-1 monocytic leukemia cells.Anti-LPAR2 (extracellular)-FITC Antibody (#ALR-032-F)

 


 

Resources

Featured Papers   Immuno-Colocalization Updates   Live Cell Imaging
Updates