Anti-mGluR1 (extracellular)-ATTO-488 Antibody

Metabotropic Glutamate receptor 1, GRM1, mGlu1
    Cat #: AGC-006-AG
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1 (Accession P23385). Extracellular, N-terminus.
    Accession (Uniprot) Number P23385
    Gene ID 24414
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human, dog - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGC-006), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic, lci
    Immunohistochemistry
    Immuno-colocalization of mGluR1 and TRPC3 in mouse cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse cerebellum sections using Anti-TRPC3-ATTO-594 Antibody (ACC-016-AR), (1:60) and Anti-mGluR1 (extracellular)-ATTO-488 Antibody (#AGC-006-AG), (1:60).  A. TRPC3 staining (red). B. mGluR1 staining (green). C. Merge of the two images suggests extensive co-localization in Purkinje cells (vertical arrows). Note expression of mGluR1 in Purkinje dendrites (horizontal arrow) but not of TRPC3. Cell nuclei are stained with DAPI (blue).
    Expression of mGluR1 in rat cerebellum
    Immunohistochemical staining of rat cerebellum frozen sections with Anti-mGluR1 (extracellular)-ATTO-488 Antibody (#AGC-006-AG) (1:20).  Staining (in green) appears in cerebellar Purkinje cells (arrows) and in the molecular layer.
    References
    1. Kew, J.N.C. and Kemp, J.A. (2005) Psychopharmacol. (Berl.) 179, 4.
    2. Ferraguti, F. and Shigemoto, R. (2006) Cell Tissue Res. 326, 428.
    3. Dhami, G.P. and Ferguson, S.S.G. (2006) Pharmacol. Ther111, 260.
    Scientific background

    L-Glutamate is the major excitatory neurotransmitter in the central nervous system.  It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

    Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.

    mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.

    mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.

    The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson’s and Huntington’s disease.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/10/2018

    Anti-mGluR1 (extracellular) Antibody (#AGC-006) is a highly specific antibody directed against the extracellular N-terminus domain of rat mGluR1. The antibody can be used in western blot analysis and immunocytochemistry applications, and recognizes rat mGluR1 in rat, mouse and human samples.

    Anti-mGluR1 (extracellular)-ATTO-488 Antibody (#AGC-006-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-mGluR1 (extracellular)-ATTO-488 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use