Anti-Beta-Synuclein Antibody

β-Synuclein, Synuclein Beta, SNCB
  • New
Cat #: APZ-036
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KEEFPTDLKPEEVA, corresponding to amino acid residues 86 - 99 of rat β-Synuclein (Accession Q63754). Intracellular, C-terminus.
    Accession (Uniprot) Number Q63754
    Gene ID 113893
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Specific for β-Synuclein. No cross-reactivity with α-Synuclein.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ih, ip
    Western blot
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1, 2. Anti-Beta-Synuclein Antibody (#APZ-036), (1:200).
    3, 4. Anti-Beta-Synuclein Antibody, preincubated with the control peptide antigen.
    References
    1. Sriwimol, W. and Limprasert, P. (2018) Biomed. Res. Int. 2018, 4503871.
    2. Popova, B. et al. (2018) Front. Mol. Neurosci. 11, 94.
    3. Gámez-Valero, A. and Beyer, K. (2018) Genes (Basel) 9, 63.
    Scientific background

    β-synuclein is a presynaptic protein that plays an important role in neuronal plasticity and synaptic vesicle regulation. The synuclein family includes three members: α, β and γ isoforms. The presynaptic proteins are detected in neocortex, hippocampus, striatum, thalamus, and cerebellum regions1,2. β-Synuclein has been shown to act as a negative regulator of α-synuclein aggregation both in vivo and in vitro1,2. Structure wise, the protein contains a highly conserved N-terminal domain which includes a repetitive amino acid motif, and a more divergent and highly acidic stretch at the C-terminal domain2,3.

    Studies have shown overexpressed mRNA levels of β-synuclein in Parkinson's disease suggesting that the protein has a valuable role in maintaining homeostasis in the disease pathology. In addition, studies have shown the action of β-synuclein as a neurodegeneration-inducing factor that can cause cell loss when expressed in rat brains1,2.

    β-synuclein is also implicated with dementia involving Lewy bodies. To date, two mutations in the protein, V70M and P123H, have been identified in the sporadic and familial disease3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/11/2018

    Anti-Beta-Synuclein Antibody (#APZ-036) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize β-Synuclein from human, mouse, and rat samples. The antibody is specific for β-Synuclein. It will not cross-react with α-Synuclein.

    For research purposes only, not for human use