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Anti-Beta-Synuclein Antibody

β-Synuclein, Synuclein beta, PNP 14, SNCB
Cat #: APZ-036
Alternative Name β-Synuclein, Synuclein beta, PNP 14, SNCB
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KEEFPTDLKPEEVA, corresponding to amino acid residues 86-99 of rat β-synuclein (Accession Q63754). Intracellular, C-terminus.
Accession (Uniprot) Number Q63754
Gene ID 113893
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756783.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Specific for β-synuclein. No cross-reactivity with α-synuclein.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-Beta-Synuclein Antibody (#APZ-036), (1:200).
    3,4. Anti-Beta-Synuclein Antibody, preincubated with Beta-Synuclein Blocking Peptide (#BLP-PZ036).
References
  1. Sriwimol, W. and Limprasert, P. (2018) Biomed. Res. Int. 2018, 4503871.
  2. Popova, B. et al. (2018) Front. Mol. Neurosci. 11, 94.
  3. Gámez-Valero, A. and Beyer, K. (2018) Genes (Basel) 9, 63.
Scientific background

β-Synuclein is a presynaptic protein that plays an important role in neuronal plasticity and synaptic vesicle regulation. The synuclein family includes three members: α, β and γ isoforms. The presynaptic proteins are detected in the neocortex, hippocampus, striatum, thalamus, and cerebellum regions1,2. β-Synuclein has been shown to act as a negative regulator of α-synuclein aggregation both in vivo and in vitro1,2. Structure wise, the protein contains a highly conserved N-terminal domain which includes a repetitive amino acid motif, and a more divergent and highly acidic stretch at the C-terminal domain2,3.

Studies have shown overexpressed mRNA levels of β-synuclein in Parkinson's disease suggesting that the protein has a valuable role in maintaining homeostasis in the disease pathology. In addition, studies have shown the action of β-synuclein as a neurodegeneration-inducing factor that can cause cell loss when expressed in rat brains1,2.

β-Synuclein is also implicated with dementia involving Lewy bodies. To date, two mutations in the protein, V70M and P123H, have been identified in the sporadic and familial disease3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Anti-Beta-Synuclein Antibody (#APZ-036) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize β-synuclein from human, mouse, and rat samples. The antibody is specific for β-synuclein. It will not cross-react with α-synuclein.

For research purposes only, not for human use
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