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Anti-CADM1/SynCAM (extracellular) Antibody

Cell Adhesion Molecule 1, Synaptic Cell Adhesion Molecule, Nectin-Like 2, NECL-2, Immunoglobulin Superfamily Member 4, IgSF4

Cat #: ANR-201
Alternative Name Cell Adhesion Molecule 1, Synaptic Cell Adhesion Molecule, Nectin-Like 2, NECL-2, Immunoglobulin Superfamily Member 4, IgSF4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RNLMIDIQKDTAVEGE, corresponding to amino acid residues 148 - 163 of mouse CADM1 (Accession Q8R5M8). Extracellular, N-term.
Accession (Uniprot) Number Q8R5M8
Gene ID 54725
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
CADM1/SynCAM (extracellular) Blocking Peptide (#BLP-NR201)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201), (1:500).
    3-4. Anti-CADM1/SynCAM (extracellular) Antibody, preincubated with CADM1/SynCAM (extracellular) Blocking Peptide (BLP-NR201).
  • Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat spleen membranes (lanes 2 and 4):
    Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat spleen membranes (lanes 2 and 4):
    1-2. Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201), (1:500).
    3-4. Anti-CADM1/SynCAM (extracellular) Antibody, preincubated with CADM1/SynCAM (extracellular) Blocking Peptide (BLP-NR201).
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
    1. Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201), (1:500).
    2. Anti-CADM1/SynCAM (extracellular) Antibody, preincubated with CADM1/SynCAM (extracellular) Blocking Peptide (BLP-NR201).
Immunohistochemistry
  • Expression of CADM1 in mouse basal forebrain.
    Expression of CADM1 in mouse basal forebrain.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CADM1 immunoreactivity (green) appears in neurons (arrows).  B. Pre-incubation of the antibody with CADM1/SynCAM (extracellular) Blocking Peptide (BLP-NR201), suppressed staining. Cell nuclei are stained with DAPI (blue).
  • Expression of CADM1 in mouse deep cerebellar nuclei.
    Expression of CADM1 in mouse deep cerebellar nuclei.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CADM1 immunoreactivity (green) appears in neurons (arrows).  B. Pre-incubation of the antibody with CADM1/SynCAM (extracellular) Blocking Peptide (BLP-NR201), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of CADM1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
    Cell surface detection of CADM1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-APC.
    ___ Cells + Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201), (5μg) + goat-anti-rabbit-APC.
References
  1. Biederer, T.et al. (2006) Genomics. 87(1): p. 139-50.
  2. Park, K.A, et al. (2016) J Neurosci. 36(28): p. 7464-75.
  3. Thomas, L.A., M.R. Akins, and T. Biederer, et al. (2008) J Comp Neurol. 510(1): p. 47-67.
  4. Ryan, T.J. and S.G. Grant, et al. (2009) Nat Rev Neurosci. 10(10): p. 701-12.
  5. Gomyo, H., et al. (1999) Genomics. 62(2): p. 139-46.
  6. Wakayama, T., et al. (2001) Mol Reprod Dev. 60(2): p. 158-64.
  7. Urase, K., et al. (2001) Neuroreport. 12(15): p. 3217-21.
  8. Kuramochi, M., et al. (2001) Nat Genet. 27(4): p. 427-30.
  9. Li, H. et al. (2021) Front. Cell Dev. Biol. 9, 714298.
  10. Ujita, E., et al. (2012) PLoS One. 7(1): p. e30151.
Scientific background

SynCAMs are a family of 4 proteins (SynCAM1-4) belonging to the immunoglobulin superfamily of cell adhesion molecules (IgCAMs) and are encoded by the cell adhesion molecule 1-4 (CADM1-4) genes1-3.

Evolutionarily, SynCAMs are a fairly recent gene lineage since they are only found in vertebrates1, in contrast to much older synaptogenic adhesion molecules such as neuroligins, which appeared concurrently with the evolution of the first synapses around one billion years ago, or compared to even older protosynaptic adhesion molecules including neurexins and NCAM4.

CADM1 (also known as SynCAM1), by far the most studied family member, was identified nearly simultaneously in multiple tissue types and therefore is known by a number of different protein names including immunoglobulin subfamily member 4 (IGSF4)5, spermatogenic immunoglobulin superfamily (SgIGSF)6, and RA1757.

CADM1 was also initially discovered as a tumor suppressor gene and termed tumor suppressor in non-small cell lung cancer (TSLC1)8. In fact, CADM1 is downregulated in several tumors including lung, ovarian, hepatocellular carcinoma and glioblastoma, and has been proposed as both a biomarker of malignancy and a therapeutic target9.

In addition, CADM1 knockout mice have impaired ultrasonic vocalization, a method for mother-offspring communication, which supports a role for CADM1 in autism spectrum disorders (ASD)10.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/11/2022

Anti-CADM1/SynCAM (extracellular) Antibody (#ANR-201) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CADM1 from mouse, rat and human samples.

For research purposes only, not for human use
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