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Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody

Voltage-dependent calcium channel subunit alpha-2/delta-2
Cat #: ACC-102
Alternative Name Voltage-dependent calcium channel subunit alpha-2/delta-2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • (C)DLEAWAEKFKVLASNR, corresponding to amino acid residues 850-865 of rat CaVα2δ2 (Accession Q8CFG6). Extracellular, N-terminus.
Accession (Uniprot) Number Q8CFG6
Gene ID 300992
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_11124467.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates:
    Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates:
    1-5. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102), (1:200).
    6-10. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody, preincubated with CACNA2D2/Cavα2δ2 (extracellular) Blocking Peptide (#BLP-CC102).
Immunohistochemistry
  • Expression of CaVα2δ2 channel in rat cerebellum
    Expression of CaVα2δ2 channel in rat cerebellum
    Immunohistochemical staining rat cerebellum using Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102). A. CaVα2δ2 (green) appears in the soma of Purkinje cells (arrow) and in the molecular layer (M). B. Preincubation of the antibody with the blocking peptide blocks staining of Purkinje cells and molecular layer. DAPI is used as the counterstain (blue).
References
  1. Bauer, C.S. et al. (2010) Curr. Opin. Neurobiol. 20, 563.
  2. Jay, S.D. et al. (1991) J. Biol. Chem. 266, 3287.
  3. Davies, A. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 1654.
  4. Klugbauer, N. et al. (2003) J. Bioenerg. Biomembr. 35, 639.
Scientific background

Voltage-gated Ca2+ channels (CaV), enable the passage of Ca2+ ions in a voltage-dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel1.

CaV1 and CaV2 channels are high-voltage activated (HVA) CaV channels. The a1 subunit of these channels normally interacts and associates with α2δ subunit, a membrane anchored protein and CaVβ, a cytosolic protein1.

Four α2δ subunits have been cloned to date: α2δ1-4. This subunit originates from a single gene. The corresponding protein is modified by post-translational cleavage yielding a α2 subunit which is disulfide bonded to the δ subunit2. All α2δ subunits are GPI- (glycosylphosphatidylinositol) anchored proteins3. The role of this subunit is important for the membrane trafficking of the α1 subunit, and also has a role in influencing the biophysical properties of the channel1.

α2δ can be expressed as various splice variants and expressed in a tissue specific manner. α2δ2 can be detected in the brain, heart, lung, spleen and liver4.

Gabapentin and pregabalin are two commonly used anti-epileptic drugs. They act on CaV channels via the α2δ1 and α2δ2 subunits by disturbing their membrane trafficking, thereby decreasing Ca2+ currents.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat CaVα2δ2. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CaVα2δ2 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat TG and dorsal Vc/C2 lysates (1:200).
    Li, K.W. et al. (2014) J. Biol. Chem. 289, 7025.
  2. Xenopus oocytes expressing α2δ2 subunit.
    Edvardson, S. et al. (2013) J. Med. Genet. 50, 118.
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