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Anti-CaVpan α1 Antibody

Cat #: ACC-004
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat CaV1.2 (Accession P22002). Intracellular, C-terminus region.
Accession (Uniprot) Number P22002
Gene ID 24239
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology CaV1 family (α1C, α1D, α1F, α1S) from all known species - identical; CaV2 family (α1A, α1B, α1E) from all known species - 17/19 amino acid residues identical.
RRID AB_2039762.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, ip, wb
May also work in: ic*, ifc*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-CaVpan α1 Antibody (#ACC-004), (1:200).
    2. Anti-CaVpan α1 Antibody, preincubated with Pan CaV α 1 Blocking Peptide (#BLP-CC004).
Immunoprecipitation
  • Rat basilar artery lysates (Murphy, K. et al. (2003) Mol. Pharmacol. 64, 640.).
Immunohistochemistry
  • Human placenta (1:300) (Bernucci, L. et al. (2006) Placenta 27, 1082.).
References
  1. Bonilla, M. and Cunningham, K.M. (2002) Sci. STKE 127, PE17.
  2. Wu, X. et al. (2004) J. Biol. Chem. 279, 43392.
  3. Iwasaki, S. et al. (2000) J. Neurosci. 20, 59.
  4. Mark, M.D. and Herlitze, S. (2000) Eur. J. Biochem. 267, 5830.
Scientific background

Ca2+ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.1,2 In neurons, Ca2+ entry through voltage-dependent Ca2+ (CaV) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca2+ channels.3

Five types of Ca2+ channels are expressed in the CNS: the L-type (CaV1), N-type (CaV2.2), P/Q-type (CaV2.1), R-type (CaV2.3), and the T-type (CaV3).4 Each Cachannel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (CaVβ), α2δ and γ subunits. There are four known CaVβ subunits, in addition to four α2δ subunits and eight γ subunits.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer an antibody directed against an epitope of rat CaV1.2 channel that is identical in all isoforms of CaV1 and CaV2. Anti-CaVpan α1 Antibody (#ACC-004) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize the high voltage-gated calcium channels, CaV1 and CaV2 subfamily members, from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse eye lysate.
    Grabner, C.P. et al. (2015) J. Neurosci. 35, 13133.
  2. Mouse heart lysates (1:400).
    Viola, H.M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E2905.
Immunoprecipitation citations
  1. Mouse heart lysates (1.2 µg).
    Viola, H.M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E2905.
  2. Rat basilar artery lysates.
    Murphy, K. et al. (2003) Mol. Pharmacol. 64, 640.
Immunohistochemistry citations
  1. Mouse eye sections.
    Grabner, C.P. et al. (2015) J. Neurosci. 35, 13133.
  2. Crab cardiac ganglion (1:100).
    Ransdell, J.L. et al. (2013) J. Neurophysiol. 110, 42.
  3. Human placenta (1:300).
    Bernucci, L. et al. (2006) Placenta 27, 1082.
More product citations
  1. Fadool, D.A. et. al. (2004) Neuron 41, 389.
  2. MacPherson, M.R. et. al. (2001) Am. J. Physiol. 280, C394.
  3. Clarke, M.S. et al. (2000) J. Appl. Physiol 89, 731.
  4. Pereon, Y. et al. (1998) Pflugers Arch. 436, 309.
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