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Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody

γ-Aminobutyric acid receptor subunit θ, GABA(A) receptor subunit theta
Cat #: AGA-018
Alternative Name γ-Aminobutyric acid receptor subunit θ, GABA(A) receptor subunit theta
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABRQ (Accession Q9JLF1). Extracellular, N-terminus.
Accession (Uniprot) Number Q9JLF1
Gene ID 57249
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human - identical.
RRID AB_2340942.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and CCF-STGI cell lysate (lanes 3 and 6):
    Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and CCF-STGI cell lysate (lanes 3 and 6):
    1-3. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:500).
    4-6. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody, preincubated with GABA(A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018).
Immunohistochemistry
  • Expression of of GABA(A) θ receptor in rat hypothalamus
    Expression of of GABA(A) θ receptor in rat hypothalamus
    Immunohistochemical staining of rat hypothalamus using Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018). A. GABRQ staining (red) is detected in the mammillary nucleus which is part of the posterior hypothalamus (arrows demarcate nucleus border). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge images of A and B.
Live cell imaging / Immunocytochemistry
  • Expression of GABA(A) θ receptor in rat PC12 cells
    Expression of GABA(A) θ receptor in rat PC12 cells
    Cell surface detection of GABRQ in intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:50), (red). B. Merge of A with the live view of the cell.
References
  1. Hevers, W. and Luddens, H. (1998) Mol. Neurobiol. 18, 35.
  2. Barnard, E.A. et al. (1998) Pharmacol. Rev. 50, 291.
  3. Sieghart, W. and Sperk, G. (2002) Curr. Top. Med. Chem. 2, 795.
  4. Wisden, W. et al. (1992) J. Neurosci. 12, 1040.
  5. Bonnert, T.P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 9891.
  6. Pinna, G. et al. (2006) Psychopharmacology (Berl) 186, 362.
Scientific background

The A-type receptors for γ-aminobutyric acid (GABA(A)) mediate the majority of fast inhibitory transmission in the mammalian central nervous system. Many clinically important drugs including benzodiazepines, barbiturates and general anesthetics act as positive allosteric modulators of this receptor1. GABA(A) receptors are pentameric ligand-gated ion-channels, composed of subunits from many subunit classes: α1-α6, β1-β4, γ1-γ4, δ, ε , θ, π and ρ1-ρ32.  The arrangement of subunits around the channel is probably γβαβα counter-clockwise when viewed from the extracellular space, ε and π subunits can replace the γ and δ subunit within the pentamer, whereas the θ subunit could replace a β subunit3.

The localized expression of this subunit is consistent with the fact that all GABA(A) receptor subunit genes have distinct expression patterns in the brain3. The θ subunit has been reported to be strongly expressed in the striatum and has been localized to the locus ceruleus4.

Impairment of the γ-aminobutyric acid (GABA) signaling system is believed to partially account for behavioral and cognitive deficits associated with schizophrenia and mood disorders5. Reduction of GABA(A) mediated signal transmission has also been associated with anxiety, panic, impaired learning and memory6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse γ-aminobutyric acid receptor subunit θ. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize GABRQ from rat, mouse and human samples.

For research purposes only, not for human use
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