- Peptide DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABRQ (Accession Q9JLF1). Extracellular, N-terminus.
- Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and Human CCF-STGI brain astrocytoma cell lysate (lanes 3 and 6):1-3. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:500).
4-6. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody, preincubated with GABA(A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018).
- Expression of of GABA(A) θ receptor in rat hypothalamusImmunohistochemical staining of rat hypothalamus using Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018). A. GABRQ staining (red) is detected in the mammillary nucleus which is part of the posterior hypothalamus (arrows demarcate nucleus border). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge images of A and B.
- Expression of GABA(A) θ receptor in rat PC12 cellsCell surface detection of GABRQ in intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:50), (red). B. Merge of A with the live view of the cell.
- Hevers, W. and Luddens, H. (1998) Mol. Neurobiol. 18, 35.
- Barnard, E.A. et al. (1998) Pharmacol. Rev. 50, 291.
- Sieghart, W. and Sperk, G. (2002) Curr. Top. Med. Chem. 2, 795.
- Wisden, W. et al. (1992) J. Neurosci. 12, 1040.
- Bonnert, T.P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 9891.
- Pinna, G. et al. (2006) Psychopharmacology (Berl) 186, 362.
The A-type receptors for γ-aminobutyric acid (GABA(A)) mediate the majority of fast inhibitory transmission in the mammalian central nervous system. Many clinically important drugs including benzodiazepines, barbiturates and general anesthetics act as positive allosteric modulators of this receptor1. GABA(A) receptors are pentameric ligand-gated ion-channels, composed of subunits from many subunit classes: α1-α6, β1-β4, γ1-γ4, δ, ε , θ, π and ρ1-ρ32. The arrangement of subunits around the channel is probably γβαβα counter-clockwise when viewed from the extracellular space, ε and π subunits can replace the γ and δ subunit within the pentamer, whereas the θ subunit could replace a β subunit3.
The localized expression of this subunit is consistent with the fact that all GABA(A) receptor subunit genes have distinct expression patterns in the brain3. The θ subunit has been reported to be strongly expressed in the striatum and has been localized to the locus ceruleus4.
Impairment of the γ-aminobutyric acid (GABA) signaling system is believed to partially account for behavioral and cognitive deficits associated with schizophrenia and mood disorders5. Reduction of GABA(A) mediated signal transmission has also been associated with anxiety, panic, impaired learning and memory6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse γ-aminobutyric acid receptor subunit θ. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize GABRQ from rat, mouse and human samples.