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Anti-GPR12 (extracellular) Antibody

G-Protein Coupled Receptor 12, GPCR21

Cat #: AGR-062
Alternative Name G-Protein Coupled Receptor 12, GPCR21
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12 (Accession P35412). Extracellular, N-term.
Accession (Uniprot) Number P35412
Gene ID 14738
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical, Human – 12 out of 13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control

1 µg peptide per 1 µg antibody
GPR12 (extracellular) Blocking Peptide (BLP-GR062)

Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-GPR12 (extracellular) Antibody (#AGR-062), (1:200).
    3-4. Anti-GPR12 (extracellular) Antibody, preincubated with GPR12 (extracellular) Blocking Peptide (BLP-GR062).
Immunohistochemistry
  • Expression of GPR12 in rat cortex.
    Expression of GPR12 in rat cortex.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR12 (extracellular) Antibody (#AGR-062), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Staining in the parietal cortex region, showed GPR12 immunoreactivity (red) in glial cell outlines (arrows). B. Pre-incubation of the antibody with GPR12 (extracellular) Blocking Peptide (BLP-GR062), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Rosenbaum, D.M. et al. (2009) Nature., 459, 356.
  2. Tang, X.I. et al. (2012) Acta. Pharmacol., Sin., 33, 36.
  3. Allende, G. et al. (2020) Front. Pharmacol., 11, 628.
  4. Laun, A.S. et al. (2019) Acta. Pharmacol. Sin., 40, 300.
Scientific background

G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs is a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.

GPR12 is an orphan G protein-coupled receptor related phylogenetically, functionally, and pharmacologically with the cannabinoid system. It considered as a “constitutively active receptor” since it greatly stimulates adenylate cyclase in the absence of ligand-receptor interaction. GPR12 seems to couple Gαs as well as Gαi proteins since it stimulates baseline cAMP-dependent signaling by activating Gαs proteins, and also inhibits forskolin activated signaling through Gαi interaction3.

GPR12 expressed in the pituitary gland, in neurons in the frontal cortex, piriform cortex, thalamus, hypothalamus, hippocampus, amygdala, and olfactory bulb. It was found that GPR12 plays a significant role in energy balance. GPR12 knock out mice exhibit increase body weight and body fat mass.  In addition, overexpression of GPR12 leads to cancer cells migration and invasion via phosphorylation of Keratin 84.

Furthermore, GPR12 has been identified as an inverse agonist of the phytocannabinoid cannabidiol (CBD)4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 26/05/2022

Anti-GPR12 (extracellular) Antibody (#AGR-062) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR12 from rat, mouse and human samples.

For research purposes only, not for human use
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